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Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids

Phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 and platelet activating factor (PAF) stimulated the generation of oxygen free radicals (nitro-blue tetrazolium reduction) in Ehrlich ascites tumour (EAT) cells. PAF was effective at an optimal concentration of 4 μM, but was inhibited by...

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Autores principales: Marathe, Gopal K., D'Souza, Cletus J. M.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365376/
https://www.ncbi.nlm.nih.gov/pubmed/18475503
http://dx.doi.org/10.1155/S0962935193000079
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author Marathe, Gopal K.
D'Souza, Cletus J. M.
author_facet Marathe, Gopal K.
D'Souza, Cletus J. M.
author_sort Marathe, Gopal K.
collection PubMed
description Phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 and platelet activating factor (PAF) stimulated the generation of oxygen free radicals (nitro-blue tetrazolium reduction) in Ehrlich ascites tumour (EAT) cells. PAF was effective at an optimal concentration of 4 μM, but was inhibited by BN 52021, a specific PAF antagonist. Lyso-PAF was ineffective. Inclusion of different lipids during incubation prior to the addition of PAF, resulted in the activation/inhibition of free radical generation. Among the phospholipids at a concentration of 50 μg/ml, the order of activation was phosphatidylserine > phosphatidylglycerol > phosphoinositides > phosphatidylinositol > phosphatidylethanolamine. Phosphatidylcholine was not effective, while sphingolipids were inhibitory. In addition, Ehrlich ascites tumour cells grown in mice under marginal vitamin A deficiency, showed an augmented production of free radicals compared to control cells. This was suppressed by exogenous addition of vitamin A or superoxide dismutase. These results suggest that membrane lipids and dietary factors like vitamin A probably function as physiological modulators in regulating the free radical generation.
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spelling pubmed-23653762008-05-12 Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids Marathe, Gopal K. D'Souza, Cletus J. M. Mediators Inflamm Research Article Phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 and platelet activating factor (PAF) stimulated the generation of oxygen free radicals (nitro-blue tetrazolium reduction) in Ehrlich ascites tumour (EAT) cells. PAF was effective at an optimal concentration of 4 μM, but was inhibited by BN 52021, a specific PAF antagonist. Lyso-PAF was ineffective. Inclusion of different lipids during incubation prior to the addition of PAF, resulted in the activation/inhibition of free radical generation. Among the phospholipids at a concentration of 50 μg/ml, the order of activation was phosphatidylserine > phosphatidylglycerol > phosphoinositides > phosphatidylinositol > phosphatidylethanolamine. Phosphatidylcholine was not effective, while sphingolipids were inhibitory. In addition, Ehrlich ascites tumour cells grown in mice under marginal vitamin A deficiency, showed an augmented production of free radicals compared to control cells. This was suppressed by exogenous addition of vitamin A or superoxide dismutase. These results suggest that membrane lipids and dietary factors like vitamin A probably function as physiological modulators in regulating the free radical generation. Hindawi Publishing Corporation 1993 /pmc/articles/PMC2365376/ /pubmed/18475503 http://dx.doi.org/10.1155/S0962935193000079 Text en Copyright © 1993 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Marathe, Gopal K.
D'Souza, Cletus J. M.
Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids
title Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids
title_full Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids
title_fullStr Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids
title_full_unstemmed Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids
title_short Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids
title_sort production of oxygen free radicals by ehrlich ascites tumour cells: effect of lipids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365376/
https://www.ncbi.nlm.nih.gov/pubmed/18475503
http://dx.doi.org/10.1155/S0962935193000079
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