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Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids
Phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 and platelet activating factor (PAF) stimulated the generation of oxygen free radicals (nitro-blue tetrazolium reduction) in Ehrlich ascites tumour (EAT) cells. PAF was effective at an optimal concentration of 4 μM, but was inhibited by...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
1993
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365376/ https://www.ncbi.nlm.nih.gov/pubmed/18475503 http://dx.doi.org/10.1155/S0962935193000079 |
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author | Marathe, Gopal K. D'Souza, Cletus J. M. |
author_facet | Marathe, Gopal K. D'Souza, Cletus J. M. |
author_sort | Marathe, Gopal K. |
collection | PubMed |
description | Phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 and platelet activating factor (PAF) stimulated the generation of oxygen free radicals (nitro-blue tetrazolium reduction) in Ehrlich ascites tumour (EAT) cells. PAF was effective at an optimal concentration of 4 μM, but was inhibited by BN 52021, a specific PAF antagonist. Lyso-PAF was ineffective. Inclusion of different lipids during incubation prior to the addition of PAF, resulted in the activation/inhibition of free radical generation. Among the phospholipids at a concentration of 50 μg/ml, the order of activation was phosphatidylserine > phosphatidylglycerol > phosphoinositides > phosphatidylinositol > phosphatidylethanolamine. Phosphatidylcholine was not effective, while sphingolipids were inhibitory. In addition, Ehrlich ascites tumour cells grown in mice under marginal vitamin A deficiency, showed an augmented production of free radicals compared to control cells. This was suppressed by exogenous addition of vitamin A or superoxide dismutase. These results suggest that membrane lipids and dietary factors like vitamin A probably function as physiological modulators in regulating the free radical generation. |
format | Text |
id | pubmed-2365376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-23653762008-05-12 Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids Marathe, Gopal K. D'Souza, Cletus J. M. Mediators Inflamm Research Article Phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 and platelet activating factor (PAF) stimulated the generation of oxygen free radicals (nitro-blue tetrazolium reduction) in Ehrlich ascites tumour (EAT) cells. PAF was effective at an optimal concentration of 4 μM, but was inhibited by BN 52021, a specific PAF antagonist. Lyso-PAF was ineffective. Inclusion of different lipids during incubation prior to the addition of PAF, resulted in the activation/inhibition of free radical generation. Among the phospholipids at a concentration of 50 μg/ml, the order of activation was phosphatidylserine > phosphatidylglycerol > phosphoinositides > phosphatidylinositol > phosphatidylethanolamine. Phosphatidylcholine was not effective, while sphingolipids were inhibitory. In addition, Ehrlich ascites tumour cells grown in mice under marginal vitamin A deficiency, showed an augmented production of free radicals compared to control cells. This was suppressed by exogenous addition of vitamin A or superoxide dismutase. These results suggest that membrane lipids and dietary factors like vitamin A probably function as physiological modulators in regulating the free radical generation. Hindawi Publishing Corporation 1993 /pmc/articles/PMC2365376/ /pubmed/18475503 http://dx.doi.org/10.1155/S0962935193000079 Text en Copyright © 1993 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Marathe, Gopal K. D'Souza, Cletus J. M. Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids |
title | Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids |
title_full | Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids |
title_fullStr | Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids |
title_full_unstemmed | Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids |
title_short | Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids |
title_sort | production of oxygen free radicals by ehrlich ascites tumour cells: effect of lipids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365376/ https://www.ncbi.nlm.nih.gov/pubmed/18475503 http://dx.doi.org/10.1155/S0962935193000079 |
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