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TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1

The role of eosinophils in inflammation and their mode of activation is not well understood. Eosinophil accumulation and subsequent expression of cytokines at the site of inflammation may play a role in exacerbation of inflammatory responses. In the present study, we have examined the role of TNF-α...

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Autores principales: Goldstein, L. A., Strieter, R. M., Evanoff, H. L., Kunkel, S. L., Lukacs, N. W.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365785/
https://www.ncbi.nlm.nih.gov/pubmed/18475720
http://dx.doi.org/10.1155/S0962935196000312
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author Goldstein, L. A.
Strieter, R. M.
Evanoff, H. L.
Kunkel, S. L.
Lukacs, N. W.
author_facet Goldstein, L. A.
Strieter, R. M.
Evanoff, H. L.
Kunkel, S. L.
Lukacs, N. W.
author_sort Goldstein, L. A.
collection PubMed
description The role of eosinophils in inflammation and their mode of activation is not well understood. Eosinophil accumulation and subsequent expression of cytokines at the site of inflammation may play a role in exacerbation of inflammatory responses. In the present study, we have examined the role of TNF-α in eosinophil activation and chemokine production using a human leukaemic eosinophil cell line, EOL-1. Initial studies demonstrated that TNF-α induced the upregulation of IL-8 and MCP-1 mRNA and protein. Kinetic studies indicated production of chemokines, IL-8 and MCP-1, as early as 4 h post-activation, with peak levels of chemokine produced at 8 h, and decreasing by 24 h post-TNF-α activation. When IL-10, a suppressive cytokine, was incubated with TNF-α and EOL-1 cells, no effect was observed on IL-8 and MCP-1 production. However, dexamethasone, a glucocorticoid, demonstrated potent inhibitory effects on the EOL-1-derived chemokines. These studies indicate that eosinophils may be a significant source of chemokines capable of participating in, and maintaining, leukocyte recruitment during inflammatory responses, such as asthma.
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spelling pubmed-23657852008-05-12 TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1 Goldstein, L. A. Strieter, R. M. Evanoff, H. L. Kunkel, S. L. Lukacs, N. W. Mediators Inflamm Research Article The role of eosinophils in inflammation and their mode of activation is not well understood. Eosinophil accumulation and subsequent expression of cytokines at the site of inflammation may play a role in exacerbation of inflammatory responses. In the present study, we have examined the role of TNF-α in eosinophil activation and chemokine production using a human leukaemic eosinophil cell line, EOL-1. Initial studies demonstrated that TNF-α induced the upregulation of IL-8 and MCP-1 mRNA and protein. Kinetic studies indicated production of chemokines, IL-8 and MCP-1, as early as 4 h post-activation, with peak levels of chemokine produced at 8 h, and decreasing by 24 h post-TNF-α activation. When IL-10, a suppressive cytokine, was incubated with TNF-α and EOL-1 cells, no effect was observed on IL-8 and MCP-1 production. However, dexamethasone, a glucocorticoid, demonstrated potent inhibitory effects on the EOL-1-derived chemokines. These studies indicate that eosinophils may be a significant source of chemokines capable of participating in, and maintaining, leukocyte recruitment during inflammatory responses, such as asthma. Hindawi Publishing Corporation 1996-06 /pmc/articles/PMC2365785/ /pubmed/18475720 http://dx.doi.org/10.1155/S0962935196000312 Text en Copyright © 1996 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Goldstein, L. A.
Strieter, R. M.
Evanoff, H. L.
Kunkel, S. L.
Lukacs, N. W.
TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1
title TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1
title_full TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1
title_fullStr TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1
title_full_unstemmed TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1
title_short TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1
title_sort tnf-induced il-8 and mcp-1 production in the eosinophilic cell line, eol-1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365785/
https://www.ncbi.nlm.nih.gov/pubmed/18475720
http://dx.doi.org/10.1155/S0962935196000312
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