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Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction

We report the regulation of type 1 receptor mRNA in Y-79 human retinoblastoma cells, grown in the absence or presence of pharmacological levels of phorbol esters, forskolin, glucocorticoids and their combinations. To control for inducibility and for assessing the sensitivity of the Y-79 system to gl...

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Detalles Bibliográficos
Autores principales: Vamvakopoulos, N. C., Sioutopoulou, T. O., Mamuris, Z., Marcoulatos, P., Avgerinos, P. C.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365798/
https://www.ncbi.nlm.nih.gov/pubmed/18475726
http://dx.doi.org/10.1155/S0962935196000476
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author Vamvakopoulos, N. C.
Sioutopoulou, T. O.
Mamuris, Z.
Marcoulatos, P.
Avgerinos, P. C.
author_facet Vamvakopoulos, N. C.
Sioutopoulou, T. O.
Mamuris, Z.
Marcoulatos, P.
Avgerinos, P. C.
author_sort Vamvakopoulos, N. C.
collection PubMed
description We report the regulation of type 1 receptor mRNA in Y-79 human retinoblastoma cells, grown in the absence or presence of pharmacological levels of phorbol esters, forskolin, glucocorticoids and their combinations. To control for inducibility and for assessing the sensitivity of the Y-79 system to glucocorticoids, corticotropin releasing hormone mRNA levels were measured in parallel. All treatments stimulated corticotropin releasing hormone receptor type 1 gene expression relative to baseline. A weak suppression of corticotropin releasing hormone mRNA level was observed during dexamethasone treatment. The cell line expressed ten-fold excess of receptor to ligand mRNA under basal conditions. The findings predict the presence of functional phorbol ester, cyclic AMP and glucocorticoid response elements in the promoter region of corticotropin releasing hormone receptor type 1 gene and support a potential role for its product during chronic stress and immune/inflammatory reaction.
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spelling pubmed-23657982008-05-12 Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction Vamvakopoulos, N. C. Sioutopoulou, T. O. Mamuris, Z. Marcoulatos, P. Avgerinos, P. C. Mediators Inflamm Research Article We report the regulation of type 1 receptor mRNA in Y-79 human retinoblastoma cells, grown in the absence or presence of pharmacological levels of phorbol esters, forskolin, glucocorticoids and their combinations. To control for inducibility and for assessing the sensitivity of the Y-79 system to glucocorticoids, corticotropin releasing hormone mRNA levels were measured in parallel. All treatments stimulated corticotropin releasing hormone receptor type 1 gene expression relative to baseline. A weak suppression of corticotropin releasing hormone mRNA level was observed during dexamethasone treatment. The cell line expressed ten-fold excess of receptor to ligand mRNA under basal conditions. The findings predict the presence of functional phorbol ester, cyclic AMP and glucocorticoid response elements in the promoter region of corticotropin releasing hormone receptor type 1 gene and support a potential role for its product during chronic stress and immune/inflammatory reaction. Hindawi Publishing Corporation 1996-10 /pmc/articles/PMC2365798/ /pubmed/18475726 http://dx.doi.org/10.1155/S0962935196000476 Text en Copyright © 1996 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vamvakopoulos, N. C.
Sioutopoulou, T. O.
Mamuris, Z.
Marcoulatos, P.
Avgerinos, P. C.
Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction
title Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction
title_full Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction
title_fullStr Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction
title_full_unstemmed Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction
title_short Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction
title_sort regulation of corticotropin releasing hormone receptor type 1 messenger rna level in y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365798/
https://www.ncbi.nlm.nih.gov/pubmed/18475726
http://dx.doi.org/10.1155/S0962935196000476
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