Cargando…
Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells
The role of the L-arginine–nitric oxide metabolic pathway was explored for interleukin-2-induced proliferation in the cytotoxic T lymphocyte clone CTLL-2. Specific inhibition of nitric oxide synthase significantly diminished, in a concentration-dependent manner, (3)H-thymidine uptake of CTLL-2 cells...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
1996
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365802/ https://www.ncbi.nlm.nih.gov/pubmed/18475725 http://dx.doi.org/10.1155/S0962935196000464 |
_version_ | 1782154220849332224 |
---|---|
author | Padrón, J. Glaría, L. Martinez, O. Torres, M. Lopez, E. Delgado, R. Caveda, L. Rojas, A. |
author_facet | Padrón, J. Glaría, L. Martinez, O. Torres, M. Lopez, E. Delgado, R. Caveda, L. Rojas, A. |
author_sort | Padrón, J. |
collection | PubMed |
description | The role of the L-arginine–nitric oxide metabolic pathway was explored for interleukin-2-induced proliferation in the cytotoxic T lymphocyte clone CTLL-2. Specific inhibition of nitric oxide synthase significantly diminished, in a concentration-dependent manner, (3)H-thymidine uptake of CTLL-2 cells in response to different concentrations of interleukin 2. Withdrawal of L-arginine from culture medium resulted as potent as the higher inhibition obtained when blocking nitric oxide synthase with L-arginine analogues. Furthermore, intermedial concentrations of Larginine and exogenous nitric oxide donors were found for achieving optimal IL2-induced proliferation of CTLL-2. These findings prompted us to suggest that intra- and/or inter-cellular nitric oxide signalling may contribute to the modulation of the IL2 mitogenic effect upon cytotoxic T lymphocytes. |
format | Text |
id | pubmed-2365802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1996 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-23658022008-05-12 Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells Padrón, J. Glaría, L. Martinez, O. Torres, M. Lopez, E. Delgado, R. Caveda, L. Rojas, A. Mediators Inflamm Research Article The role of the L-arginine–nitric oxide metabolic pathway was explored for interleukin-2-induced proliferation in the cytotoxic T lymphocyte clone CTLL-2. Specific inhibition of nitric oxide synthase significantly diminished, in a concentration-dependent manner, (3)H-thymidine uptake of CTLL-2 cells in response to different concentrations of interleukin 2. Withdrawal of L-arginine from culture medium resulted as potent as the higher inhibition obtained when blocking nitric oxide synthase with L-arginine analogues. Furthermore, intermedial concentrations of Larginine and exogenous nitric oxide donors were found for achieving optimal IL2-induced proliferation of CTLL-2. These findings prompted us to suggest that intra- and/or inter-cellular nitric oxide signalling may contribute to the modulation of the IL2 mitogenic effect upon cytotoxic T lymphocytes. Hindawi Publishing Corporation 1996-10 /pmc/articles/PMC2365802/ /pubmed/18475725 http://dx.doi.org/10.1155/S0962935196000464 Text en Copyright © 1996 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Padrón, J. Glaría, L. Martinez, O. Torres, M. Lopez, E. Delgado, R. Caveda, L. Rojas, A. Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
title | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
title_full | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
title_fullStr | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
title_full_unstemmed | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
title_short | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
title_sort | nitric oxide modulates interleukin-2-induced proliferation in ctll-2 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365802/ https://www.ncbi.nlm.nih.gov/pubmed/18475725 http://dx.doi.org/10.1155/S0962935196000464 |
work_keys_str_mv | AT padronj nitricoxidemodulatesinterleukin2inducedproliferationinctll2cells AT glarial nitricoxidemodulatesinterleukin2inducedproliferationinctll2cells AT martinezo nitricoxidemodulatesinterleukin2inducedproliferationinctll2cells AT torresm nitricoxidemodulatesinterleukin2inducedproliferationinctll2cells AT lopeze nitricoxidemodulatesinterleukin2inducedproliferationinctll2cells AT delgador nitricoxidemodulatesinterleukin2inducedproliferationinctll2cells AT cavedal nitricoxidemodulatesinterleukin2inducedproliferationinctll2cells AT rojasa nitricoxidemodulatesinterleukin2inducedproliferationinctll2cells |