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Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein
Lsr2 is a small, basic protein present in Mycobacterium and related actinomycetes. Recent studies suggest that Lsr2 is a regulatory protein involved in multiple cellular processes including cell wall biosynthesis and antibiotic resistance. However, the underlying molecular mechanisms remain unknown....
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2367712/ https://www.ncbi.nlm.nih.gov/pubmed/18187505 http://dx.doi.org/10.1093/nar/gkm1162 |
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author | Chen, Jeffrey M. Ren, Huiping Shaw, James E. Wang, Yu Jing Li, Ming Leung, Andrea S. Tran, Vanessa Berbenetz, Nicolas M. Kocíncová, Dana Yip, Christopher M. Reyrat, Jean-Marc Liu, Jun |
author_facet | Chen, Jeffrey M. Ren, Huiping Shaw, James E. Wang, Yu Jing Li, Ming Leung, Andrea S. Tran, Vanessa Berbenetz, Nicolas M. Kocíncová, Dana Yip, Christopher M. Reyrat, Jean-Marc Liu, Jun |
author_sort | Chen, Jeffrey M. |
collection | PubMed |
description | Lsr2 is a small, basic protein present in Mycobacterium and related actinomycetes. Recent studies suggest that Lsr2 is a regulatory protein involved in multiple cellular processes including cell wall biosynthesis and antibiotic resistance. However, the underlying molecular mechanisms remain unknown. In this article, we performed biochemical studies of Lsr2–DNA interactions and structure–function analysis of Lsr2. Analysis by atomic force microscopy revealed that Lsr2 has the ability to bridge distant DNA segments, suggesting that Lsr2 plays a role in the overall organization and compactness of the nucleoid. Mutational analysis identified critical residues and selection of dominant negative mutants demonstrated that both DNA binding and protein oligomerization are essential for the normal functions of Lsr2 in vivo. These results provide strong evidence that Lsr2 is a DNA bridging protein, which represents the first identification of such proteins in bacteria phylogenetically distant from the Enterobacteriaceae. DNA bridging by Lsr2 also provides a mechanism of transcriptional regulation by Lsr2. |
format | Text |
id | pubmed-2367712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-23677122008-05-07 Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein Chen, Jeffrey M. Ren, Huiping Shaw, James E. Wang, Yu Jing Li, Ming Leung, Andrea S. Tran, Vanessa Berbenetz, Nicolas M. Kocíncová, Dana Yip, Christopher M. Reyrat, Jean-Marc Liu, Jun Nucleic Acids Res Molecular Biology Lsr2 is a small, basic protein present in Mycobacterium and related actinomycetes. Recent studies suggest that Lsr2 is a regulatory protein involved in multiple cellular processes including cell wall biosynthesis and antibiotic resistance. However, the underlying molecular mechanisms remain unknown. In this article, we performed biochemical studies of Lsr2–DNA interactions and structure–function analysis of Lsr2. Analysis by atomic force microscopy revealed that Lsr2 has the ability to bridge distant DNA segments, suggesting that Lsr2 plays a role in the overall organization and compactness of the nucleoid. Mutational analysis identified critical residues and selection of dominant negative mutants demonstrated that both DNA binding and protein oligomerization are essential for the normal functions of Lsr2 in vivo. These results provide strong evidence that Lsr2 is a DNA bridging protein, which represents the first identification of such proteins in bacteria phylogenetically distant from the Enterobacteriaceae. DNA bridging by Lsr2 also provides a mechanism of transcriptional regulation by Lsr2. Oxford University Press 2008-04 2008-01-10 /pmc/articles/PMC2367712/ /pubmed/18187505 http://dx.doi.org/10.1093/nar/gkm1162 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Molecular Biology Chen, Jeffrey M. Ren, Huiping Shaw, James E. Wang, Yu Jing Li, Ming Leung, Andrea S. Tran, Vanessa Berbenetz, Nicolas M. Kocíncová, Dana Yip, Christopher M. Reyrat, Jean-Marc Liu, Jun Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein |
title | Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein |
title_full | Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein |
title_fullStr | Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein |
title_full_unstemmed | Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein |
title_short | Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein |
title_sort | lsr2 of mycobacterium tuberculosis is a dna-bridging protein |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2367712/ https://www.ncbi.nlm.nih.gov/pubmed/18187505 http://dx.doi.org/10.1093/nar/gkm1162 |
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