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In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system

BACKGROUND: Due to its extremely high strength, the interaction between biotin and (strept)avidin has been exploited for a large number of biotechnological applications. Site-specific biotinylation of proteins in vivo can be achieved by co-expressing in mammalian cells the protein of interest fused...

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Detalles Bibliográficos
Autores principales: Predonzani, Andrea, Arnoldi, Francesca, López-Requena, Alejandro, Burrone, Oscar R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373293/
https://www.ncbi.nlm.nih.gov/pubmed/18423015
http://dx.doi.org/10.1186/1472-6750-8-41