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In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system
BACKGROUND: Due to its extremely high strength, the interaction between biotin and (strept)avidin has been exploited for a large number of biotechnological applications. Site-specific biotinylation of proteins in vivo can be achieved by co-expressing in mammalian cells the protein of interest fused...
Autores principales: | Predonzani, Andrea, Arnoldi, Francesca, López-Requena, Alejandro, Burrone, Oscar R |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373293/ https://www.ncbi.nlm.nih.gov/pubmed/18423015 http://dx.doi.org/10.1186/1472-6750-8-41 |
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