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Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases
We describe a new approach for labeling of unique sequences within dsDNA under nondenaturing conditions. The method is based on the site-specific formation of vicinal nicks, which are created by nicking endonucleases (NEases) at specified DNA sites on the same strand within dsDNA. The oligomeric seg...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373456/ https://www.ncbi.nlm.nih.gov/pubmed/18344522 http://dx.doi.org/10.1093/nar/gkn107 |