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IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8(+) T cells

We propose a novel role for interleukin (IL) 6 in inducing rapid spontaneous proliferation (SP) of naive CD8(+) T cells, which is a crucial step in the differentiation of colitogenic CD8(+) T cells. Homeostasis of T cells is regulated by two distinct modes of cell proliferation: major histocompatibi...

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Autores principales: Tajima, Masaki, Wakita, Daiko, Noguchi, Daisuke, Chamoto, Kenji, Yue, Zhang, Fugo, Kazunori, Ishigame, Harumichi, Iwakura, Yoichiro, Kitamura, Hidemitsu, Nishimura, Takashi
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373835/
https://www.ncbi.nlm.nih.gov/pubmed/18426983
http://dx.doi.org/10.1084/jem.20071133
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author Tajima, Masaki
Wakita, Daiko
Noguchi, Daisuke
Chamoto, Kenji
Yue, Zhang
Fugo, Kazunori
Ishigame, Harumichi
Iwakura, Yoichiro
Kitamura, Hidemitsu
Nishimura, Takashi
author_facet Tajima, Masaki
Wakita, Daiko
Noguchi, Daisuke
Chamoto, Kenji
Yue, Zhang
Fugo, Kazunori
Ishigame, Harumichi
Iwakura, Yoichiro
Kitamura, Hidemitsu
Nishimura, Takashi
author_sort Tajima, Masaki
collection PubMed
description We propose a novel role for interleukin (IL) 6 in inducing rapid spontaneous proliferation (SP) of naive CD8(+) T cells, which is a crucial step in the differentiation of colitogenic CD8(+) T cells. Homeostasis of T cells is regulated by two distinct modes of cell proliferation: major histocompatibility complex/antigen–driven rapid SP and IL-7/IL-15–dependent slow homeostatic proliferation. Using our novel model of CD8(+) T cell–dependent colitis, we found that SP of naive CD8(+) T cells is essential for inducing pathogenic cytokine-producing effector T cells. The rapid SP was predominantly induced in mesenteric lymph nodes (LNs) but not in peripheral LNs under the influence of intestinal flora and IL-6. Indeed, this SP was markedly inhibited by treatment with anti–IL-6 receptor monoclonal antibody (IL-6R mAb) or antibiotic-induced flora depletion, but not by anti–IL-7R mAb and/or in IL-15–deficient conditions. Concomitantly with the inhibition of SP, anti–IL-6R mAb significantly inhibited the induction of CD8(+) T cell–dependent autoimmune colitis. Notably, the transfer of naive CD8(+) T cells derived from IL-17(−/−) mice did not induce autoimmune colitis. Thus, we conclude that IL-6 signaling is crucial for SP under lymphopenic conditions, which subsequently caused severe IL-17–producing CD8(+) T cell–mediated autoimmune colitis. We suggest that anti–IL-6R mAb may become a promising strategy for the therapy of colitis.
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spelling pubmed-23738352008-11-12 IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8(+) T cells Tajima, Masaki Wakita, Daiko Noguchi, Daisuke Chamoto, Kenji Yue, Zhang Fugo, Kazunori Ishigame, Harumichi Iwakura, Yoichiro Kitamura, Hidemitsu Nishimura, Takashi J Exp Med Brief Definitive Reports We propose a novel role for interleukin (IL) 6 in inducing rapid spontaneous proliferation (SP) of naive CD8(+) T cells, which is a crucial step in the differentiation of colitogenic CD8(+) T cells. Homeostasis of T cells is regulated by two distinct modes of cell proliferation: major histocompatibility complex/antigen–driven rapid SP and IL-7/IL-15–dependent slow homeostatic proliferation. Using our novel model of CD8(+) T cell–dependent colitis, we found that SP of naive CD8(+) T cells is essential for inducing pathogenic cytokine-producing effector T cells. The rapid SP was predominantly induced in mesenteric lymph nodes (LNs) but not in peripheral LNs under the influence of intestinal flora and IL-6. Indeed, this SP was markedly inhibited by treatment with anti–IL-6 receptor monoclonal antibody (IL-6R mAb) or antibiotic-induced flora depletion, but not by anti–IL-7R mAb and/or in IL-15–deficient conditions. Concomitantly with the inhibition of SP, anti–IL-6R mAb significantly inhibited the induction of CD8(+) T cell–dependent autoimmune colitis. Notably, the transfer of naive CD8(+) T cells derived from IL-17(−/−) mice did not induce autoimmune colitis. Thus, we conclude that IL-6 signaling is crucial for SP under lymphopenic conditions, which subsequently caused severe IL-17–producing CD8(+) T cell–mediated autoimmune colitis. We suggest that anti–IL-6R mAb may become a promising strategy for the therapy of colitis. The Rockefeller University Press 2008-05-12 /pmc/articles/PMC2373835/ /pubmed/18426983 http://dx.doi.org/10.1084/jem.20071133 Text en © 2008 Tajima et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Brief Definitive Reports
Tajima, Masaki
Wakita, Daiko
Noguchi, Daisuke
Chamoto, Kenji
Yue, Zhang
Fugo, Kazunori
Ishigame, Harumichi
Iwakura, Yoichiro
Kitamura, Hidemitsu
Nishimura, Takashi
IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8(+) T cells
title IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8(+) T cells
title_full IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8(+) T cells
title_fullStr IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8(+) T cells
title_full_unstemmed IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8(+) T cells
title_short IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8(+) T cells
title_sort il-6–dependent spontaneous proliferation is required for the induction of colitogenic il-17–producing cd8(+) t cells
topic Brief Definitive Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373835/
https://www.ncbi.nlm.nih.gov/pubmed/18426983
http://dx.doi.org/10.1084/jem.20071133
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