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Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs
It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. We have used this approach to generate novel influenza vaccines that fuse the globular head domain of the protective hemagglutinin (HA) antigen with the potent TLR5...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373928/ https://www.ncbi.nlm.nih.gov/pubmed/18493310 http://dx.doi.org/10.1371/journal.pone.0002257 |
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author | Song, Langzhou Nakaar, Valerian Kavita, Uma Price, Albert Huleatt, Jim Tang, Jie Jacobs, Andrea Liu, Ge Huang, Yan Desai, Priyanka Maksymiuk, Gail Takahashi, Virginia Umlauf, Scott Reiserova, Lucia Bell, Rodney Li, Hong Zhang, Yi McDonald, William F. Powell, T. J. Tussey, Lynda |
author_facet | Song, Langzhou Nakaar, Valerian Kavita, Uma Price, Albert Huleatt, Jim Tang, Jie Jacobs, Andrea Liu, Ge Huang, Yan Desai, Priyanka Maksymiuk, Gail Takahashi, Virginia Umlauf, Scott Reiserova, Lucia Bell, Rodney Li, Hong Zhang, Yi McDonald, William F. Powell, T. J. Tussey, Lynda |
author_sort | Song, Langzhou |
collection | PubMed |
description | It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. We have used this approach to generate novel influenza vaccines that fuse the globular head domain of the protective hemagglutinin (HA) antigen with the potent TLR5 ligand, flagellin. These fusion proteins are efficiently expressed in standard E. coli fermentation systems and the HA moiety can be faithfully refolded to take on the native conformation of the globular head. In mouse models of influenza infection, the vaccines elicit robust antibody responses that mitigate disease and protect mice from lethal challenge. These immunologically potent vaccines can be efficiently manufactured to support pandemic response, pre-pandemic and seasonal vaccines. |
format | Text |
id | pubmed-2373928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-23739282008-05-21 Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs Song, Langzhou Nakaar, Valerian Kavita, Uma Price, Albert Huleatt, Jim Tang, Jie Jacobs, Andrea Liu, Ge Huang, Yan Desai, Priyanka Maksymiuk, Gail Takahashi, Virginia Umlauf, Scott Reiserova, Lucia Bell, Rodney Li, Hong Zhang, Yi McDonald, William F. Powell, T. J. Tussey, Lynda PLoS One Research Article It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. We have used this approach to generate novel influenza vaccines that fuse the globular head domain of the protective hemagglutinin (HA) antigen with the potent TLR5 ligand, flagellin. These fusion proteins are efficiently expressed in standard E. coli fermentation systems and the HA moiety can be faithfully refolded to take on the native conformation of the globular head. In mouse models of influenza infection, the vaccines elicit robust antibody responses that mitigate disease and protect mice from lethal challenge. These immunologically potent vaccines can be efficiently manufactured to support pandemic response, pre-pandemic and seasonal vaccines. Public Library of Science 2008-05-21 /pmc/articles/PMC2373928/ /pubmed/18493310 http://dx.doi.org/10.1371/journal.pone.0002257 Text en Song et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Song, Langzhou Nakaar, Valerian Kavita, Uma Price, Albert Huleatt, Jim Tang, Jie Jacobs, Andrea Liu, Ge Huang, Yan Desai, Priyanka Maksymiuk, Gail Takahashi, Virginia Umlauf, Scott Reiserova, Lucia Bell, Rodney Li, Hong Zhang, Yi McDonald, William F. Powell, T. J. Tussey, Lynda Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs |
title | Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs |
title_full | Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs |
title_fullStr | Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs |
title_full_unstemmed | Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs |
title_short | Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs |
title_sort | efficacious recombinant influenza vaccines produced by high yield bacterial expression: a solution to global pandemic and seasonal needs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373928/ https://www.ncbi.nlm.nih.gov/pubmed/18493310 http://dx.doi.org/10.1371/journal.pone.0002257 |
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