Cargando…
Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature
Since microvascular dysfunction with complete circulatory arrest and, thus, prolongation of tissue ischaemia is considered a potential mechanism for cell necrosis following hepatic cryosurgery, we determined the temperature necessary for induction of complete nutritive perfusion failure in cryotherm...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2000
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374393/ https://www.ncbi.nlm.nih.gov/pubmed/10732748 http://dx.doi.org/10.1054/bjoc.1999.1001 |
_version_ | 1782154442910466048 |
---|---|
author | Schüder, G Pistorius, G Fehringer, M Feifel, G Menger, M D Vollmar, B |
author_facet | Schüder, G Pistorius, G Fehringer, M Feifel, G Menger, M D Vollmar, B |
author_sort | Schüder, G |
collection | PubMed |
description | Since microvascular dysfunction with complete circulatory arrest and, thus, prolongation of tissue ischaemia is considered a potential mechanism for cell necrosis following hepatic cryosurgery, we determined the temperature necessary for induction of complete nutritive perfusion failure in cryothermia-treated rat livers. After localization of the cryoprobe with seven thermocouples and application of a single or double freeze–thaw cycle, in vivo fluorescence microscopy of the cryoinjured left lobe was performed over a 2-h period using a computer-controlled stepping motor, which guaranteed analysis of the identical liver tissue segments with exact allocation of the thermocouples and thus determination of tissue temperature. Cryothermia resulted in a central non-perfused part of injury, surrounded by a heterogeneously perfused peripheral zone. The non-perfused area after single and double freezing continuously increased over the first 90-min period due to a successive shutdown of perfusion within the peripheral border zone. Analysis of the thermocouples' temperature at the end of freezing revealed the 0°C-front at 11.7 mm (single freeze–thaw cycle) and 12.1 mm (double freeze–thaw cycle) distant from the centre of the cryoprobe, which exactly corresponds with the initial (30 min) expansion of the area with nutritive perfusion failure. The increased non-perfused tissue area at 2 h conformed a critical border temperature between 8.29 ± 1.63°C and 9.07 ± 0.24°C. From these findings, we conclude that freezing of liver tissue to temperatures of at least < 0°C causes complete/irreversible perfusion failure, which consequently will result in cell death and tissue necrosis, and may thus be supposed as a prerequisite for the safe and successful application of cryosurgery in hepatic tumour ablation. © 2000 Cancer Research Campaign |
format | Text |
id | pubmed-2374393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23743932009-09-10 Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature Schüder, G Pistorius, G Fehringer, M Feifel, G Menger, M D Vollmar, B Br J Cancer Regular Article Since microvascular dysfunction with complete circulatory arrest and, thus, prolongation of tissue ischaemia is considered a potential mechanism for cell necrosis following hepatic cryosurgery, we determined the temperature necessary for induction of complete nutritive perfusion failure in cryothermia-treated rat livers. After localization of the cryoprobe with seven thermocouples and application of a single or double freeze–thaw cycle, in vivo fluorescence microscopy of the cryoinjured left lobe was performed over a 2-h period using a computer-controlled stepping motor, which guaranteed analysis of the identical liver tissue segments with exact allocation of the thermocouples and thus determination of tissue temperature. Cryothermia resulted in a central non-perfused part of injury, surrounded by a heterogeneously perfused peripheral zone. The non-perfused area after single and double freezing continuously increased over the first 90-min period due to a successive shutdown of perfusion within the peripheral border zone. Analysis of the thermocouples' temperature at the end of freezing revealed the 0°C-front at 11.7 mm (single freeze–thaw cycle) and 12.1 mm (double freeze–thaw cycle) distant from the centre of the cryoprobe, which exactly corresponds with the initial (30 min) expansion of the area with nutritive perfusion failure. The increased non-perfused tissue area at 2 h conformed a critical border temperature between 8.29 ± 1.63°C and 9.07 ± 0.24°C. From these findings, we conclude that freezing of liver tissue to temperatures of at least < 0°C causes complete/irreversible perfusion failure, which consequently will result in cell death and tissue necrosis, and may thus be supposed as a prerequisite for the safe and successful application of cryosurgery in hepatic tumour ablation. © 2000 Cancer Research Campaign Nature Publishing Group 2000-02 /pmc/articles/PMC2374393/ /pubmed/10732748 http://dx.doi.org/10.1054/bjoc.1999.1001 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Regular Article Schüder, G Pistorius, G Fehringer, M Feifel, G Menger, M D Vollmar, B Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature |
title | Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature |
title_full | Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature |
title_fullStr | Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature |
title_full_unstemmed | Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature |
title_short | Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature |
title_sort | complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374393/ https://www.ncbi.nlm.nih.gov/pubmed/10732748 http://dx.doi.org/10.1054/bjoc.1999.1001 |
work_keys_str_mv | AT schuderg completeshutdownofmicrovascularperfusionuponhepaticcryothermiaiscriticallydependentonlocaltissuetemperature AT pistoriusg completeshutdownofmicrovascularperfusionuponhepaticcryothermiaiscriticallydependentonlocaltissuetemperature AT fehringerm completeshutdownofmicrovascularperfusionuponhepaticcryothermiaiscriticallydependentonlocaltissuetemperature AT feifelg completeshutdownofmicrovascularperfusionuponhepaticcryothermiaiscriticallydependentonlocaltissuetemperature AT mengermd completeshutdownofmicrovascularperfusionuponhepaticcryothermiaiscriticallydependentonlocaltissuetemperature AT vollmarb completeshutdownofmicrovascularperfusionuponhepaticcryothermiaiscriticallydependentonlocaltissuetemperature |