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Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma

Neuroblastoma is the commonest extracranial solid tumour in children. There are a number of molecular genetic features known which are of prognostic importance and which are used to direct therapy. Identification and targeting of high-risk individuals with intensive therapeutic regimens may allow an...

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Autores principales: Taylor, C P F, Bown, N P, McGuckin, A G, Lunec, J, Malcolm, A J, Pearson, A D J, Sheer, D
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374533/
https://www.ncbi.nlm.nih.gov/pubmed/10883666
http://dx.doi.org/10.1054/bjoc.2000.1280
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author Taylor, C P F
Bown, N P
McGuckin, A G
Lunec, J
Malcolm, A J
Pearson, A D J
Sheer, D
author_facet Taylor, C P F
Bown, N P
McGuckin, A G
Lunec, J
Malcolm, A J
Pearson, A D J
Sheer, D
author_sort Taylor, C P F
collection PubMed
description Neuroblastoma is the commonest extracranial solid tumour in children. There are a number of molecular genetic features known which are of prognostic importance and which are used to direct therapy. Identification and targeting of high-risk individuals with intensive therapeutic regimens may allow an improvement in survival rates. The most powerful biological parameters associated with prognosis in this malignancy are chromosomal changes, especially MYCN amplification, deletion of chromosome 1p and aneuploidy. Rapid characterization of these aberrations at the time of diagnosis is paramount if stratification according to risk group is to be achieved. This paper describes the rapid detection of del(1p), MYCN amplification and trisomy using interphase fluorescence in situ hybridization on imprints from fresh tumour biopsies. The results are related to those obtained by standard molecular methods and karyotyping. © 2000 Cancer Research Campaign
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spelling pubmed-23745332009-09-10 Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma Taylor, C P F Bown, N P McGuckin, A G Lunec, J Malcolm, A J Pearson, A D J Sheer, D Br J Cancer Regular Article Neuroblastoma is the commonest extracranial solid tumour in children. There are a number of molecular genetic features known which are of prognostic importance and which are used to direct therapy. Identification and targeting of high-risk individuals with intensive therapeutic regimens may allow an improvement in survival rates. The most powerful biological parameters associated with prognosis in this malignancy are chromosomal changes, especially MYCN amplification, deletion of chromosome 1p and aneuploidy. Rapid characterization of these aberrations at the time of diagnosis is paramount if stratification according to risk group is to be achieved. This paper describes the rapid detection of del(1p), MYCN amplification and trisomy using interphase fluorescence in situ hybridization on imprints from fresh tumour biopsies. The results are related to those obtained by standard molecular methods and karyotyping. © 2000 Cancer Research Campaign Nature Publishing Group 2000-07 2000-06-02 /pmc/articles/PMC2374533/ /pubmed/10883666 http://dx.doi.org/10.1054/bjoc.2000.1280 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Taylor, C P F
Bown, N P
McGuckin, A G
Lunec, J
Malcolm, A J
Pearson, A D J
Sheer, D
Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma
title Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma
title_full Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma
title_fullStr Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma
title_full_unstemmed Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma
title_short Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma
title_sort fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374533/
https://www.ncbi.nlm.nih.gov/pubmed/10883666
http://dx.doi.org/10.1054/bjoc.2000.1280
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