UTILLdb, a Pisum sativum in silico forward and reverse genetics tool

The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population an...

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Detalles Bibliográficos
Autores principales: Dalmais, Marion, Schmidt, Julien, Le Signor, Christine, Moussy, Francoise, Burstin, Judith, Savois, Vincent, Aubert, Gregoire, Brunaud, Veronique, de Oliveira, Yannick, Guichard, Cecile, Thompson, Richard, Bendahmane, Abdelhafid
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374714/
https://www.ncbi.nlm.nih.gov/pubmed/18302733
http://dx.doi.org/10.1186/gb-2008-9-2-r43
Descripción
Sumario:The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.

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