UTILLdb, a Pisum sativum in silico forward and reverse genetics tool

The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population an...

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Autores principales: Dalmais, Marion, Schmidt, Julien, Le Signor, Christine, Moussy, Francoise, Burstin, Judith, Savois, Vincent, Aubert, Gregoire, Brunaud, Veronique, de Oliveira, Yannick, Guichard, Cecile, Thompson, Richard, Bendahmane, Abdelhafid
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374714/
https://www.ncbi.nlm.nih.gov/pubmed/18302733
http://dx.doi.org/10.1186/gb-2008-9-2-r43
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author Dalmais, Marion
Schmidt, Julien
Le Signor, Christine
Moussy, Francoise
Burstin, Judith
Savois, Vincent
Aubert, Gregoire
Brunaud, Veronique
de Oliveira, Yannick
Guichard, Cecile
Thompson, Richard
Bendahmane, Abdelhafid
author_facet Dalmais, Marion
Schmidt, Julien
Le Signor, Christine
Moussy, Francoise
Burstin, Judith
Savois, Vincent
Aubert, Gregoire
Brunaud, Veronique
de Oliveira, Yannick
Guichard, Cecile
Thompson, Richard
Bendahmane, Abdelhafid
author_sort Dalmais, Marion
collection PubMed
description The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.
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spelling pubmed-23747142008-05-09 UTILLdb, a Pisum sativum in silico forward and reverse genetics tool Dalmais, Marion Schmidt, Julien Le Signor, Christine Moussy, Francoise Burstin, Judith Savois, Vincent Aubert, Gregoire Brunaud, Veronique de Oliveira, Yannick Guichard, Cecile Thompson, Richard Bendahmane, Abdelhafid Genome Biol Method The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords. BioMed Central 2008 2008-02-26 /pmc/articles/PMC2374714/ /pubmed/18302733 http://dx.doi.org/10.1186/gb-2008-9-2-r43 Text en Copyright © 2008 Dalmais et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Dalmais, Marion
Schmidt, Julien
Le Signor, Christine
Moussy, Francoise
Burstin, Judith
Savois, Vincent
Aubert, Gregoire
Brunaud, Veronique
de Oliveira, Yannick
Guichard, Cecile
Thompson, Richard
Bendahmane, Abdelhafid
UTILLdb, a Pisum sativum in silico forward and reverse genetics tool
title UTILLdb, a Pisum sativum in silico forward and reverse genetics tool
title_full UTILLdb, a Pisum sativum in silico forward and reverse genetics tool
title_fullStr UTILLdb, a Pisum sativum in silico forward and reverse genetics tool
title_full_unstemmed UTILLdb, a Pisum sativum in silico forward and reverse genetics tool
title_short UTILLdb, a Pisum sativum in silico forward and reverse genetics tool
title_sort utilldb, a pisum sativum in silico forward and reverse genetics tool
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374714/
https://www.ncbi.nlm.nih.gov/pubmed/18302733
http://dx.doi.org/10.1186/gb-2008-9-2-r43
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