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Stable Isotope Analysis Can Potentially Identify Completely-Digested Bloodmeals in Mosquitoes
BACKGROUND: Vertebrate bloodfeeding is a critical component of a mosquito's ability to transmit pathogens that cause diseases such as malaria, dengue fever and viral encephalitis. Due to degradation by the digestive process, current methods to identify mosquito bloodmeal sources are only useful...
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374900/ https://www.ncbi.nlm.nih.gov/pubmed/18493314 http://dx.doi.org/10.1371/journal.pone.0002198 |
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author | Rasgon, Jason L. |
author_facet | Rasgon, Jason L. |
author_sort | Rasgon, Jason L. |
collection | PubMed |
description | BACKGROUND: Vertebrate bloodfeeding is a critical component of a mosquito's ability to transmit pathogens that cause diseases such as malaria, dengue fever and viral encephalitis. Due to degradation by the digestive process, current methods to identify mosquito bloodmeal sources are only useful for approximately 36 hours post-feeding. A critical need exists for technologies to extend this window and gain a more complete picture of mosquito feeding behavior for epidemiological studies. Stable isotopes are useful for investigating organism feeding behavior because the isotopic ratio of an organism's tissues reflects that of the material it ingests. METHODOLOGY/PRINCIPAL FINDINGS: Proof-of-principle data indicates that after bloodfeeding, Aedes albopictus mosquitoes acquire diagnostic Carbon and Nitrogen stable isotope profiles from their vertebrate hosts that can be accurately identified one week post-feeding, approximately 4 days after the entire bloodmeal has been digested. Total C/N ratio served as a biomarker marker for bloodfeeding (P<0.02), while δN was the most informative variable which could distinguish between unfed, chicken-fed and human-fed mosquitoes (P<0.01). By plotting C/N vs. δN, all feeding treatments could be identified in a double-blind analysis. CONCLUSIONS/SIGNIFICANCE: These proof-of-principle experiments indicate that analysis of stable isotopes can be used to distinguish bloodfed from unfed mosquitoes, and also distinguish between different vertebrate bloodmeal sources even after all blood has been digested. The development of stable isotope-based assays for mosquito bloodmeal identification may be a powerful tool to investigate mosquito feeding ecology and the dynamics of vector-borne pathogens. |
format | Text |
id | pubmed-2374900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-23749002008-05-21 Stable Isotope Analysis Can Potentially Identify Completely-Digested Bloodmeals in Mosquitoes Rasgon, Jason L. PLoS One Research Article BACKGROUND: Vertebrate bloodfeeding is a critical component of a mosquito's ability to transmit pathogens that cause diseases such as malaria, dengue fever and viral encephalitis. Due to degradation by the digestive process, current methods to identify mosquito bloodmeal sources are only useful for approximately 36 hours post-feeding. A critical need exists for technologies to extend this window and gain a more complete picture of mosquito feeding behavior for epidemiological studies. Stable isotopes are useful for investigating organism feeding behavior because the isotopic ratio of an organism's tissues reflects that of the material it ingests. METHODOLOGY/PRINCIPAL FINDINGS: Proof-of-principle data indicates that after bloodfeeding, Aedes albopictus mosquitoes acquire diagnostic Carbon and Nitrogen stable isotope profiles from their vertebrate hosts that can be accurately identified one week post-feeding, approximately 4 days after the entire bloodmeal has been digested. Total C/N ratio served as a biomarker marker for bloodfeeding (P<0.02), while δN was the most informative variable which could distinguish between unfed, chicken-fed and human-fed mosquitoes (P<0.01). By plotting C/N vs. δN, all feeding treatments could be identified in a double-blind analysis. CONCLUSIONS/SIGNIFICANCE: These proof-of-principle experiments indicate that analysis of stable isotopes can be used to distinguish bloodfed from unfed mosquitoes, and also distinguish between different vertebrate bloodmeal sources even after all blood has been digested. The development of stable isotope-based assays for mosquito bloodmeal identification may be a powerful tool to investigate mosquito feeding ecology and the dynamics of vector-borne pathogens. Public Library of Science 2008-05-21 /pmc/articles/PMC2374900/ /pubmed/18493314 http://dx.doi.org/10.1371/journal.pone.0002198 Text en Jason Rasgon. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rasgon, Jason L. Stable Isotope Analysis Can Potentially Identify Completely-Digested Bloodmeals in Mosquitoes |
title | Stable Isotope Analysis Can Potentially Identify Completely-Digested Bloodmeals in Mosquitoes |
title_full | Stable Isotope Analysis Can Potentially Identify Completely-Digested Bloodmeals in Mosquitoes |
title_fullStr | Stable Isotope Analysis Can Potentially Identify Completely-Digested Bloodmeals in Mosquitoes |
title_full_unstemmed | Stable Isotope Analysis Can Potentially Identify Completely-Digested Bloodmeals in Mosquitoes |
title_short | Stable Isotope Analysis Can Potentially Identify Completely-Digested Bloodmeals in Mosquitoes |
title_sort | stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374900/ https://www.ncbi.nlm.nih.gov/pubmed/18493314 http://dx.doi.org/10.1371/journal.pone.0002198 |
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