Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin

The goal of this study was to identify changes in mRNA levels in tumour cells after a toxic exposure to cisplatin (IC(99)dose). Using suppression-subtractive hybridization (SSH) 2 cDNA libraries were created, an UP library (202 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed...

Descripción completa

Detalles Bibliográficos
Autores principales: Johnsson, A, Byrne, P, Bruin, R de, Weiner, D, Wong, J, Los, G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375161/
https://www.ncbi.nlm.nih.gov/pubmed/11710836
http://dx.doi.org/10.1054/bjoc.2001.2080
_version_ 1782154591252512768
author Johnsson, A
Byrne, P
Bruin, R de
Weiner, D
Wong, J
Los, G
author_facet Johnsson, A
Byrne, P
Bruin, R de
Weiner, D
Wong, J
Los, G
author_sort Johnsson, A
collection PubMed
description The goal of this study was to identify changes in mRNA levels in tumour cells after a toxic exposure to cisplatin (IC(99)dose). Using suppression-subtractive hybridization (SSH) 2 cDNA libraries were created, an UP library (202 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed Northern hybridization 16 and 30 fragments were truly differentially expressed in the UP and DOWN libraries, respectively. Most prominent in the UP library were the mitochondrial and injury response clusters and in the DOWN library the cytoskeletal, protein synthesis and signalling clusters. These distinct clusters potentially represent an expression profile of the cisplatin-induced cellular injury response. © 2001 Cancer Research Campaign  http://www.bjcancer.com
format Text
id pubmed-2375161
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-23751612009-09-10 Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin Johnsson, A Byrne, P Bruin, R de Weiner, D Wong, J Los, G Br J Cancer Short Communication The goal of this study was to identify changes in mRNA levels in tumour cells after a toxic exposure to cisplatin (IC(99)dose). Using suppression-subtractive hybridization (SSH) 2 cDNA libraries were created, an UP library (202 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed Northern hybridization 16 and 30 fragments were truly differentially expressed in the UP and DOWN libraries, respectively. Most prominent in the UP library were the mitochondrial and injury response clusters and in the DOWN library the cytoskeletal, protein synthesis and signalling clusters. These distinct clusters potentially represent an expression profile of the cisplatin-induced cellular injury response. © 2001 Cancer Research Campaign  http://www.bjcancer.com Nature Publishing Group 2001-10 /pmc/articles/PMC2375161/ /pubmed/11710836 http://dx.doi.org/10.1054/bjoc.2001.2080 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Short Communication
Johnsson, A
Byrne, P
Bruin, R de
Weiner, D
Wong, J
Los, G
Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin
title Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin
title_full Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin
title_fullStr Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin
title_full_unstemmed Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin
title_short Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin
title_sort identification of gene clusters differentially expressed during the cellular injury responses (cir) to cisplatin
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375161/
https://www.ncbi.nlm.nih.gov/pubmed/11710836
http://dx.doi.org/10.1054/bjoc.2001.2080
work_keys_str_mv AT johnssona identificationofgeneclustersdifferentiallyexpressedduringthecellularinjuryresponsescirtocisplatin
AT byrnep identificationofgeneclustersdifferentiallyexpressedduringthecellularinjuryresponsescirtocisplatin
AT bruinrde identificationofgeneclustersdifferentiallyexpressedduringthecellularinjuryresponsescirtocisplatin
AT weinerd identificationofgeneclustersdifferentiallyexpressedduringthecellularinjuryresponsescirtocisplatin
AT wongj identificationofgeneclustersdifferentiallyexpressedduringthecellularinjuryresponsescirtocisplatin
AT losg identificationofgeneclustersdifferentiallyexpressedduringthecellularinjuryresponsescirtocisplatin

Ejemplares similares