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Differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring
Fine needle aspiration (FNA) biopsy is the least invasive method of sampling breast cancer in vivo and provides material for breast cancer diagnosis. FNA has also been used to examine cellular markers to predict and monitor the effects of therapy. The aim of this study was to assess the accuracy of...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2001
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375165/ https://www.ncbi.nlm.nih.gov/pubmed/11710820 http://dx.doi.org/10.1054/bjoc.2001.2064 |
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author | Ball, H M-L Hupp, T R Ziyaie, D Purdie, C A Kernohan, N M Thompson, A M |
author_facet | Ball, H M-L Hupp, T R Ziyaie, D Purdie, C A Kernohan, N M Thompson, A M |
author_sort | Ball, H M-L |
collection | PubMed |
description | Fine needle aspiration (FNA) biopsy is the least invasive method of sampling breast cancer in vivo and provides material for breast cancer diagnosis. FNA has also been used to examine cellular markers to predict and monitor the effects of therapy. The aim of this study was to assess the accuracy of using FNA material compared with resected cancer for Western blotting studies of the p53 pathway, a key to tumour response to radiotherapy and chemotherapy. Paired samples of breast cancer FNAs collected pre-operatively and post-operatively were compared with tissue samples obtained at the time of surgical resection. Western blots were probed for p53 using the antibodies DO12 and DO1, and for levels of downstream proteins p21/WAF1 and p27. The protein extracted by FNA was sufficient for up to 5 Western blot studies. p53 expression and phosphorylation did not differ significantly pre- and post-operatively, indicating that intra-operative manipulation does not affect p53 expression or downstream activation in breast cancer. However, expression of p53, p21 and p27 varied between individual patients suggesting a range of p53 pathway activation in breast cancer. Immunohistochemistry confirmed that the cancer cells accounted for the protein expression detected on Western blots. FNA yields adequate protein for Western blotting studies and could be used as a method to monitor p53 activity in vivo before and during anti-cancer treatment possibly providing early evidence of tumour response to therapy. © 2001 Cancer Research Campaign http://www.bjcancer.com |
format | Text |
id | pubmed-2375165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23751652009-09-10 Differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring Ball, H M-L Hupp, T R Ziyaie, D Purdie, C A Kernohan, N M Thompson, A M Br J Cancer Regular Article Fine needle aspiration (FNA) biopsy is the least invasive method of sampling breast cancer in vivo and provides material for breast cancer diagnosis. FNA has also been used to examine cellular markers to predict and monitor the effects of therapy. The aim of this study was to assess the accuracy of using FNA material compared with resected cancer for Western blotting studies of the p53 pathway, a key to tumour response to radiotherapy and chemotherapy. Paired samples of breast cancer FNAs collected pre-operatively and post-operatively were compared with tissue samples obtained at the time of surgical resection. Western blots were probed for p53 using the antibodies DO12 and DO1, and for levels of downstream proteins p21/WAF1 and p27. The protein extracted by FNA was sufficient for up to 5 Western blot studies. p53 expression and phosphorylation did not differ significantly pre- and post-operatively, indicating that intra-operative manipulation does not affect p53 expression or downstream activation in breast cancer. However, expression of p53, p21 and p27 varied between individual patients suggesting a range of p53 pathway activation in breast cancer. Immunohistochemistry confirmed that the cancer cells accounted for the protein expression detected on Western blots. FNA yields adequate protein for Western blotting studies and could be used as a method to monitor p53 activity in vivo before and during anti-cancer treatment possibly providing early evidence of tumour response to therapy. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-10 /pmc/articles/PMC2375165/ /pubmed/11710820 http://dx.doi.org/10.1054/bjoc.2001.2064 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Regular Article Ball, H M-L Hupp, T R Ziyaie, D Purdie, C A Kernohan, N M Thompson, A M Differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring |
title | Differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring |
title_full | Differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring |
title_fullStr | Differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring |
title_full_unstemmed | Differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring |
title_short | Differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring |
title_sort | differential p53 protein expression in breast cancer fine needle aspirates: the potential for in vivo monitoring |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375165/ https://www.ncbi.nlm.nih.gov/pubmed/11710820 http://dx.doi.org/10.1054/bjoc.2001.2064 |
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