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Reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas

Ovarian adenocarcinomas develop as the result of multiple genetic and epigenetic changes in the precursor ovarian surface epithelial (OSE) cells which result in a malignant phenotype. We investigated changes in gene expression in ovarian adenocarcinoma using a cDNA array containing 588 known human g...

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Autores principales: Arnold, J M, Cummings, M, Purdie, D, Chenevix-Trench, G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375262/
https://www.ncbi.nlm.nih.gov/pubmed/11720474
http://dx.doi.org/10.1054/bjoc.2001.2075
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author Arnold, J M
Cummings, M
Purdie, D
Chenevix-Trench, G
author_facet Arnold, J M
Cummings, M
Purdie, D
Chenevix-Trench, G
author_sort Arnold, J M
collection PubMed
description Ovarian adenocarcinomas develop as the result of multiple genetic and epigenetic changes in the precursor ovarian surface epithelial (OSE) cells which result in a malignant phenotype. We investigated changes in gene expression in ovarian adenocarcinoma using a cDNA array containing 588 known human genes. We found that intercellular adhesion molecule-1 (ICAM-1) was expressed at lower levels in the ovarian tumour cell lines OAW42, PEO1 and JAM than in the immortalised human ovarian surface epithelial cell line HOSE 17.1. Further investigation revealed ICAM-1 was expressed in the surface epithelium of normal ovaries and both mRNA and protein expression levels were reduced in the majority of ovarian adenocarcinoma cell lines and primary tumours. ICAM-1 expression was increased in 8/8 cell lines treated with the de novo methyltransferase inhibitor 5-aza-2′-deoxycytidine, indicating that methylation of CpG islands may play a role in the down-regulation of its expression in primary tumours. There was a significant association between patients whose tumours expressed ICAM-1 and survival (P= 0.03), suggesting that expression levels of ICAM-1 may have clinical relevance. © 2001 Cancer Research Campaign
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spelling pubmed-23752622009-09-10 Reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas Arnold, J M Cummings, M Purdie, D Chenevix-Trench, G Br J Cancer Regular Article Ovarian adenocarcinomas develop as the result of multiple genetic and epigenetic changes in the precursor ovarian surface epithelial (OSE) cells which result in a malignant phenotype. We investigated changes in gene expression in ovarian adenocarcinoma using a cDNA array containing 588 known human genes. We found that intercellular adhesion molecule-1 (ICAM-1) was expressed at lower levels in the ovarian tumour cell lines OAW42, PEO1 and JAM than in the immortalised human ovarian surface epithelial cell line HOSE 17.1. Further investigation revealed ICAM-1 was expressed in the surface epithelium of normal ovaries and both mRNA and protein expression levels were reduced in the majority of ovarian adenocarcinoma cell lines and primary tumours. ICAM-1 expression was increased in 8/8 cell lines treated with the de novo methyltransferase inhibitor 5-aza-2′-deoxycytidine, indicating that methylation of CpG islands may play a role in the down-regulation of its expression in primary tumours. There was a significant association between patients whose tumours expressed ICAM-1 and survival (P= 0.03), suggesting that expression levels of ICAM-1 may have clinical relevance. © 2001 Cancer Research Campaign Nature Publishing Group 2001-11 2001-09-01 /pmc/articles/PMC2375262/ /pubmed/11720474 http://dx.doi.org/10.1054/bjoc.2001.2075 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Arnold, J M
Cummings, M
Purdie, D
Chenevix-Trench, G
Reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas
title Reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas
title_full Reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas
title_fullStr Reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas
title_full_unstemmed Reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas
title_short Reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas
title_sort reduced expression of intercellular adhesion molecule-1 in ovarian adenocarcinomas
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375262/
https://www.ncbi.nlm.nih.gov/pubmed/11720474
http://dx.doi.org/10.1054/bjoc.2001.2075
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