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Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1
Control of translation initiation was recognised as a critical checkpoint for cell proliferation and tumorigenesis. In human melanoma cells, we have previously reported consistent overexpression of translation initiation factor eIF-4A1. Here, we investigated by transfection of antisense constructs i...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375438/ https://www.ncbi.nlm.nih.gov/pubmed/12085193 http://dx.doi.org/10.1038/sj.bjc.6600351 |
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author | Eberle, J Fecker, L F Bittner, J-U Orfanos, C E Geilen, C C |
author_facet | Eberle, J Fecker, L F Bittner, J-U Orfanos, C E Geilen, C C |
author_sort | Eberle, J |
collection | PubMed |
description | Control of translation initiation was recognised as a critical checkpoint for cell proliferation and tumorigenesis. In human melanoma cells, we have previously reported consistent overexpression of translation initiation factor eIF-4A1. Here, we investigated by transfection of antisense constructs its significance for the control of melanoma cell growth. The tetracycline-inducible expression system was established in melanoma cells, and three fragments of the 5′-, central-, and 3′-portion of the eIF-4A1 cDNA were subcloned in antisense and in sense orientation after a tetracycline inducible promoter. Significant proliferation decrease was obtained after transient transfection and induction of antisense RNA directed against the 5′- and the central portion (up to 10%), whereas, no effects were seen after induction of the 3′-fragment and the sense controls. Cell clones stably transfected with the central antisense fragment revealed after doxycycline induction reduced expression of endogeneous eIF-4A1 mRNA correlated with decreased proliferation rates (up to 6%). These data demonstrate the applicability of antisense strategies against translation factors in melanoma cells. Translation initiation factor eIF-4A1 contributes to the control of melanoma cell proliferation and may be taken into consideration when scheduling new therapeutic approaches targeting the translational control. British Journal of Cancer (2002) 86, 1957–1962. doi:10.1038/sj.bjc.6600351 www.bjcancer.com © 2002 Cancer Research UK |
format | Text |
id | pubmed-2375438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23754382009-09-10 Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1 Eberle, J Fecker, L F Bittner, J-U Orfanos, C E Geilen, C C Br J Cancer Experimental Therapeutics Control of translation initiation was recognised as a critical checkpoint for cell proliferation and tumorigenesis. In human melanoma cells, we have previously reported consistent overexpression of translation initiation factor eIF-4A1. Here, we investigated by transfection of antisense constructs its significance for the control of melanoma cell growth. The tetracycline-inducible expression system was established in melanoma cells, and three fragments of the 5′-, central-, and 3′-portion of the eIF-4A1 cDNA were subcloned in antisense and in sense orientation after a tetracycline inducible promoter. Significant proliferation decrease was obtained after transient transfection and induction of antisense RNA directed against the 5′- and the central portion (up to 10%), whereas, no effects were seen after induction of the 3′-fragment and the sense controls. Cell clones stably transfected with the central antisense fragment revealed after doxycycline induction reduced expression of endogeneous eIF-4A1 mRNA correlated with decreased proliferation rates (up to 6%). These data demonstrate the applicability of antisense strategies against translation factors in melanoma cells. Translation initiation factor eIF-4A1 contributes to the control of melanoma cell proliferation and may be taken into consideration when scheduling new therapeutic approaches targeting the translational control. British Journal of Cancer (2002) 86, 1957–1962. doi:10.1038/sj.bjc.6600351 www.bjcancer.com © 2002 Cancer Research UK Nature Publishing Group 2002-06-17 /pmc/articles/PMC2375438/ /pubmed/12085193 http://dx.doi.org/10.1038/sj.bjc.6600351 Text en Copyright © 2002 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Experimental Therapeutics Eberle, J Fecker, L F Bittner, J-U Orfanos, C E Geilen, C C Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1 |
title | Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1 |
title_full | Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1 |
title_fullStr | Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1 |
title_full_unstemmed | Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1 |
title_short | Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1 |
title_sort | decreased proliferation of human melanoma cell lines caused by antisense rna against translation factor eif-4a1 |
topic | Experimental Therapeutics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375438/ https://www.ncbi.nlm.nih.gov/pubmed/12085193 http://dx.doi.org/10.1038/sj.bjc.6600351 |
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