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Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer
The effect of selective and non-selective cyclo-oxygenase inhibition on tumour growth and metastasis in an orthotopic model of breast cancer was investigated. 4T1 mammary adenocarcinoma cells were injected into the mammary fat pad of female BALB/c mice. When tumours reached a mean tumour diameter of...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2376100/ https://www.ncbi.nlm.nih.gov/pubmed/12107848 http://dx.doi.org/10.1038/sj.bjc.6600462 |
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author | Connolly, E M Harmey, J H O'Grady, T Foley, D Roche-Nagle, G Kay, E Bouchier-Hayes, D J |
author_facet | Connolly, E M Harmey, J H O'Grady, T Foley, D Roche-Nagle, G Kay, E Bouchier-Hayes, D J |
author_sort | Connolly, E M |
collection | PubMed |
description | The effect of selective and non-selective cyclo-oxygenase inhibition on tumour growth and metastasis in an orthotopic model of breast cancer was investigated. 4T1 mammary adenocarcinoma cells were injected into the mammary fat pad of female BALB/c mice. When tumours reached a mean tumour diameter of 8.4±0.4 mm, mice were randomised into three groups (n=6 per group) and received daily intraperitoneal injections of the selective cyclo-oxygenase-2 inhibitor, SC-236, the non selective cyclo-oxygenase inhibitor, Indomethacin, or drug vehicle. Tumour diameter was recorded on alternate days. From 8 days after initiation of treatment, tumour diameter in animals treated with either SC-236 or indomethacin was significantly reduced relative to controls. Both primary tumour weight and the number of lung metastases were significantly reduced in the SC-236 and indomethacin treated mice. Microvessel density was reduced and tumor cell apoptosis increased in the primary tumour of mice treated with either the selective or non-selective cyclo-oxygenase inhibitor. In vitro, cyclo-oxygenase inhibition decreased vascular endothelial growth factor production and increased apoptosis of tumour cells. Our results suggest that cyclo-oxygenase inhibitors will be of value in the treatment of both primary and metastatic breast cancer. British Journal of Cancer (2002) 87, 231–237. doi:10.1038/sj.bjc.6600462 www.bjcancer.com © 2002 Cancer Research UK |
format | Text |
id | pubmed-2376100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23761002009-09-10 Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer Connolly, E M Harmey, J H O'Grady, T Foley, D Roche-Nagle, G Kay, E Bouchier-Hayes, D J Br J Cancer Experimental Therapeutics The effect of selective and non-selective cyclo-oxygenase inhibition on tumour growth and metastasis in an orthotopic model of breast cancer was investigated. 4T1 mammary adenocarcinoma cells were injected into the mammary fat pad of female BALB/c mice. When tumours reached a mean tumour diameter of 8.4±0.4 mm, mice were randomised into three groups (n=6 per group) and received daily intraperitoneal injections of the selective cyclo-oxygenase-2 inhibitor, SC-236, the non selective cyclo-oxygenase inhibitor, Indomethacin, or drug vehicle. Tumour diameter was recorded on alternate days. From 8 days after initiation of treatment, tumour diameter in animals treated with either SC-236 or indomethacin was significantly reduced relative to controls. Both primary tumour weight and the number of lung metastases were significantly reduced in the SC-236 and indomethacin treated mice. Microvessel density was reduced and tumor cell apoptosis increased in the primary tumour of mice treated with either the selective or non-selective cyclo-oxygenase inhibitor. In vitro, cyclo-oxygenase inhibition decreased vascular endothelial growth factor production and increased apoptosis of tumour cells. Our results suggest that cyclo-oxygenase inhibitors will be of value in the treatment of both primary and metastatic breast cancer. British Journal of Cancer (2002) 87, 231–237. doi:10.1038/sj.bjc.6600462 www.bjcancer.com © 2002 Cancer Research UK Nature Publishing Group 2002-07-15 2002-07-02 /pmc/articles/PMC2376100/ /pubmed/12107848 http://dx.doi.org/10.1038/sj.bjc.6600462 Text en Copyright © 2002 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Experimental Therapeutics Connolly, E M Harmey, J H O'Grady, T Foley, D Roche-Nagle, G Kay, E Bouchier-Hayes, D J Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer |
title | Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer |
title_full | Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer |
title_fullStr | Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer |
title_full_unstemmed | Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer |
title_short | Cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer |
title_sort | cyclo-oxygenase inhibition reduces tumour growth and metastasis in an orthotopic model of breast cancer |
topic | Experimental Therapeutics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2376100/ https://www.ncbi.nlm.nih.gov/pubmed/12107848 http://dx.doi.org/10.1038/sj.bjc.6600462 |
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