δ-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters

δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system i...

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Autores principales: Bermúdez Moretti, M, Correa García, S, Perotti, C, Batlle, A, Casas, A
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2002
Materias:
Experimental Therapeutics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2376128/
https://www.ncbi.nlm.nih.gov/pubmed/12177786
http://dx.doi.org/10.1038/sj.bjc.6600481
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author Bermúdez Moretti, M
Correa García, S
Perotti, C
Batlle, A
Casas, A
author_facet Bermúdez Moretti, M
Correa García, S
Perotti, C
Batlle, A
Casas, A
author_sort Bermúdez Moretti, M
collection PubMed
description δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using (14)C-δ-aminolevulinic acid, to finally improve δ-aminolevulinic acid incorporation in mammalian cells. Our results showed that δ-aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in δ-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2. British Journal of Cancer (2002) 87, 471–474. doi:10.1038/sj.bjc.6600481 www.bjcancer.com © 2002 Cancer Research UK
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spelling pubmed-23761282009-09-10 δ-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters Bermúdez Moretti, M Correa García, S Perotti, C Batlle, A Casas, A Br J Cancer Experimental Therapeutics δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using (14)C-δ-aminolevulinic acid, to finally improve δ-aminolevulinic acid incorporation in mammalian cells. Our results showed that δ-aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in δ-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2. British Journal of Cancer (2002) 87, 471–474. doi:10.1038/sj.bjc.6600481 www.bjcancer.com © 2002 Cancer Research UK Nature Publishing Group 2002-08-12 /pmc/articles/PMC2376128/ /pubmed/12177786 http://dx.doi.org/10.1038/sj.bjc.6600481 Text en Copyright © 2002 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Experimental Therapeutics
Bermúdez Moretti, M
Correa García, S
Perotti, C
Batlle, A
Casas, A
δ-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title δ-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_full δ-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_fullStr δ-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_full_unstemmed δ-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_short δ-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_sort δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
topic Experimental Therapeutics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2376128/
https://www.ncbi.nlm.nih.gov/pubmed/12177786
http://dx.doi.org/10.1038/sj.bjc.6600481
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