Cargando…

Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee

BACKGROUND: Chimpanzee dental pulp stem/stromal cells (ChDPSCs) are very similar to human bone marrow derived mesenchymal stem/stromal cells (hBMSCs) as demonstrated by the expression pattern of cell surface markers and their multipotent differentiation capability. RESULTS: ChDPSCs were isolated fro...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheng, Pei-Hsun, Snyder, Brooke, Fillos, Dimitri, Ibegbu, Chris C, Huang, Anderson Hsien-Cheng, Chan, Anthony WS
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2383882/
https://www.ncbi.nlm.nih.gov/pubmed/18430234
http://dx.doi.org/10.1186/1471-2121-9-20
_version_ 1782154825609248768
author Cheng, Pei-Hsun
Snyder, Brooke
Fillos, Dimitri
Ibegbu, Chris C
Huang, Anderson Hsien-Cheng
Chan, Anthony WS
author_facet Cheng, Pei-Hsun
Snyder, Brooke
Fillos, Dimitri
Ibegbu, Chris C
Huang, Anderson Hsien-Cheng
Chan, Anthony WS
author_sort Cheng, Pei-Hsun
collection PubMed
description BACKGROUND: Chimpanzee dental pulp stem/stromal cells (ChDPSCs) are very similar to human bone marrow derived mesenchymal stem/stromal cells (hBMSCs) as demonstrated by the expression pattern of cell surface markers and their multipotent differentiation capability. RESULTS: ChDPSCs were isolated from an incisor and a canine of a forty-seven year old female chimpanzee. A homogenous population of ChDPSCs was established in early culture at a high proliferation rate and verified by the expression pattern of thirteen cell surface markers. The ChDPSCs are multipotent and were capable of differentiating into osteogenic, adipogenic and chondrogenic lineages under appropriate in vitro culture conditions. ChDPSCs also express stem cell (Sox-2, Nanog, Rex-1, Oct-4) and osteogenic (Osteonectin, osteocalcin, osteopontin) markers, which is comparable to reported results of rhesus monkey BMSCs (rBMSCs), hBMSCs and hDPSCs. Although ChDPSCs vigorously proliferated during the initial phase and gradually decreased in subsequent passages, the telomere length indicated that telomerase activity was not significantly reduced. CONCLUSION: These results demonstrate that ChDPSCs can be efficiently isolated from post-mortem teeth of adult chimpanzees and are multipotent. Due to the almost identical genome composition of humans and chimpanzees, there is an emergent need for defining the new role of chimpanzee modeling in comparative medicine. Teeth are easy to recover at necropsy and easy to preserve prior to the retrieval of dental pulp for stem/stromal cells isolation. Therefore, the establishment of ChDPSCs would preserve and maximize the applications of such a unique and invaluable animal model, and could advance the understanding of cellular functions and differentiation control of adult stem cells in higher primates.
format Text
id pubmed-2383882
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-23838822008-05-14 Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee Cheng, Pei-Hsun Snyder, Brooke Fillos, Dimitri Ibegbu, Chris C Huang, Anderson Hsien-Cheng Chan, Anthony WS BMC Cell Biol Research Article BACKGROUND: Chimpanzee dental pulp stem/stromal cells (ChDPSCs) are very similar to human bone marrow derived mesenchymal stem/stromal cells (hBMSCs) as demonstrated by the expression pattern of cell surface markers and their multipotent differentiation capability. RESULTS: ChDPSCs were isolated from an incisor and a canine of a forty-seven year old female chimpanzee. A homogenous population of ChDPSCs was established in early culture at a high proliferation rate and verified by the expression pattern of thirteen cell surface markers. The ChDPSCs are multipotent and were capable of differentiating into osteogenic, adipogenic and chondrogenic lineages under appropriate in vitro culture conditions. ChDPSCs also express stem cell (Sox-2, Nanog, Rex-1, Oct-4) and osteogenic (Osteonectin, osteocalcin, osteopontin) markers, which is comparable to reported results of rhesus monkey BMSCs (rBMSCs), hBMSCs and hDPSCs. Although ChDPSCs vigorously proliferated during the initial phase and gradually decreased in subsequent passages, the telomere length indicated that telomerase activity was not significantly reduced. CONCLUSION: These results demonstrate that ChDPSCs can be efficiently isolated from post-mortem teeth of adult chimpanzees and are multipotent. Due to the almost identical genome composition of humans and chimpanzees, there is an emergent need for defining the new role of chimpanzee modeling in comparative medicine. Teeth are easy to recover at necropsy and easy to preserve prior to the retrieval of dental pulp for stem/stromal cells isolation. Therefore, the establishment of ChDPSCs would preserve and maximize the applications of such a unique and invaluable animal model, and could advance the understanding of cellular functions and differentiation control of adult stem cells in higher primates. BioMed Central 2008-04-22 /pmc/articles/PMC2383882/ /pubmed/18430234 http://dx.doi.org/10.1186/1471-2121-9-20 Text en Copyright © 2008 Cheng et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cheng, Pei-Hsun
Snyder, Brooke
Fillos, Dimitri
Ibegbu, Chris C
Huang, Anderson Hsien-Cheng
Chan, Anthony WS
Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
title Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
title_full Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
title_fullStr Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
title_full_unstemmed Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
title_short Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
title_sort postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2383882/
https://www.ncbi.nlm.nih.gov/pubmed/18430234
http://dx.doi.org/10.1186/1471-2121-9-20
work_keys_str_mv AT chengpeihsun postnatalstemprogenitorcellsderivedfromthedentalpulpofadultchimpanzee
AT snyderbrooke postnatalstemprogenitorcellsderivedfromthedentalpulpofadultchimpanzee
AT fillosdimitri postnatalstemprogenitorcellsderivedfromthedentalpulpofadultchimpanzee
AT ibegbuchrisc postnatalstemprogenitorcellsderivedfromthedentalpulpofadultchimpanzee
AT huangandersonhsiencheng postnatalstemprogenitorcellsderivedfromthedentalpulpofadultchimpanzee
AT chananthonyws postnatalstemprogenitorcellsderivedfromthedentalpulpofadultchimpanzee