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Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating
Fus2p is a pheromone-induced protein associated with the amphiphysin homologue Rvs161p, which is required for cell fusion during mating in Saccharomyces cerevisiae. We constructed a functional Fus2p–green fluorescent protein (GFP), which exhibits highly dynamic localization patterns in pheromone-res...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386108/ https://www.ncbi.nlm.nih.gov/pubmed/18474625 http://dx.doi.org/10.1083/jcb.200801101 |
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author | Paterson, Joanna Mathis Ydenberg, Casey A. Rose, Mark D. |
author_facet | Paterson, Joanna Mathis Ydenberg, Casey A. Rose, Mark D. |
author_sort | Paterson, Joanna Mathis |
collection | PubMed |
description | Fus2p is a pheromone-induced protein associated with the amphiphysin homologue Rvs161p, which is required for cell fusion during mating in Saccharomyces cerevisiae. We constructed a functional Fus2p–green fluorescent protein (GFP), which exhibits highly dynamic localization patterns in pheromone-responding cells (shmoos): diffuse nuclear, mobile cytoplasmic dots and stable cortical patches concentrated at the shmoo tip. In mitotic cells, Fus2p-GFP is nuclear but becomes cytoplasmic as cells form shmoos, dependent on the Fus3p protein kinase and high levels of pheromone signaling. The rapid cytoplasmic movement of Fus2p-GFP dots requires Rvs161p and polymerized actin and is aberrant in mutants with compromised actin organization, which suggests that the Fus2p dots are transported along actin cables, possibly in association with vesicles. Maintenance of Fus2p-GFP patches at the shmoo tip cortex is jointly dependent on actin and a membrane protein, Fus1p, which suggests that Fus1p is an anchor for Fus2p. In zygotes, Fus2p-GFP forms a dilating ring at the cell junction, returning to the nucleus at the completion of cell fusion. |
format | Text |
id | pubmed-2386108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-23861082008-11-19 Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating Paterson, Joanna Mathis Ydenberg, Casey A. Rose, Mark D. J Cell Biol Research Articles Fus2p is a pheromone-induced protein associated with the amphiphysin homologue Rvs161p, which is required for cell fusion during mating in Saccharomyces cerevisiae. We constructed a functional Fus2p–green fluorescent protein (GFP), which exhibits highly dynamic localization patterns in pheromone-responding cells (shmoos): diffuse nuclear, mobile cytoplasmic dots and stable cortical patches concentrated at the shmoo tip. In mitotic cells, Fus2p-GFP is nuclear but becomes cytoplasmic as cells form shmoos, dependent on the Fus3p protein kinase and high levels of pheromone signaling. The rapid cytoplasmic movement of Fus2p-GFP dots requires Rvs161p and polymerized actin and is aberrant in mutants with compromised actin organization, which suggests that the Fus2p dots are transported along actin cables, possibly in association with vesicles. Maintenance of Fus2p-GFP patches at the shmoo tip cortex is jointly dependent on actin and a membrane protein, Fus1p, which suggests that Fus1p is an anchor for Fus2p. In zygotes, Fus2p-GFP forms a dilating ring at the cell junction, returning to the nucleus at the completion of cell fusion. The Rockefeller University Press 2008-05-19 /pmc/articles/PMC2386108/ /pubmed/18474625 http://dx.doi.org/10.1083/jcb.200801101 Text en © 2008 Paterson et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Paterson, Joanna Mathis Ydenberg, Casey A. Rose, Mark D. Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating |
title | Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating |
title_full | Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating |
title_fullStr | Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating |
title_full_unstemmed | Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating |
title_short | Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating |
title_sort | dynamic localization of yeast fus2p to an expanding ring at the cell fusion junction during mating |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386108/ https://www.ncbi.nlm.nih.gov/pubmed/18474625 http://dx.doi.org/10.1083/jcb.200801101 |
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