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The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes

BACKGROUND: The regulation of the chloroplast antioxidant capacity depends on nuclear gene expression. For the 2-Cys peroxiredoxin-A gene (2CPA) a cis-regulatory element was recently characterized, which responds to photosynthetic redox signals. RESULTS: In a yeast-one-hybrid screen for cis-regulato...

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Autores principales: Shaikhali, Jehad, Heiber, Isabelle, Seidel, Thorsten, Ströher, Elke, Hiltscher, Heiko, Birkmann, Stefan, Dietz, Karl-Josef, Baier, Margarete
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386467/
https://www.ncbi.nlm.nih.gov/pubmed/18439303
http://dx.doi.org/10.1186/1471-2229-8-48
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author Shaikhali, Jehad
Heiber, Isabelle
Seidel, Thorsten
Ströher, Elke
Hiltscher, Heiko
Birkmann, Stefan
Dietz, Karl-Josef
Baier, Margarete
author_facet Shaikhali, Jehad
Heiber, Isabelle
Seidel, Thorsten
Ströher, Elke
Hiltscher, Heiko
Birkmann, Stefan
Dietz, Karl-Josef
Baier, Margarete
author_sort Shaikhali, Jehad
collection PubMed
description BACKGROUND: The regulation of the chloroplast antioxidant capacity depends on nuclear gene expression. For the 2-Cys peroxiredoxin-A gene (2CPA) a cis-regulatory element was recently characterized, which responds to photosynthetic redox signals. RESULTS: In a yeast-one-hybrid screen for cis-regulatory binding proteins, the transcription factor Rap2.4a was isolated. Rap2.4a controls the transcript abundance of the prominent chloroplast antioxidant enzyme through binding to the CGCG core of a CE3-like element. Rap2.4a activity is regulated by dithiol/disulfide transition of regulatory cysteinyl residues and subsequent changes in the quaternary structure. The mid-point redox potential of Rap2.4a activation is -269 mV (pH 7.0). CONCLUSION: The redox sensitivity of Rap2.4a establishes an efficient switch mechanism for redox control of nuclear gene activity of chloroplast antioxidants, in which Rap2.4 is a redox-sensor and a transducer of redox information.
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spelling pubmed-23864672008-05-16 The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes Shaikhali, Jehad Heiber, Isabelle Seidel, Thorsten Ströher, Elke Hiltscher, Heiko Birkmann, Stefan Dietz, Karl-Josef Baier, Margarete BMC Plant Biol Research Article BACKGROUND: The regulation of the chloroplast antioxidant capacity depends on nuclear gene expression. For the 2-Cys peroxiredoxin-A gene (2CPA) a cis-regulatory element was recently characterized, which responds to photosynthetic redox signals. RESULTS: In a yeast-one-hybrid screen for cis-regulatory binding proteins, the transcription factor Rap2.4a was isolated. Rap2.4a controls the transcript abundance of the prominent chloroplast antioxidant enzyme through binding to the CGCG core of a CE3-like element. Rap2.4a activity is regulated by dithiol/disulfide transition of regulatory cysteinyl residues and subsequent changes in the quaternary structure. The mid-point redox potential of Rap2.4a activation is -269 mV (pH 7.0). CONCLUSION: The redox sensitivity of Rap2.4a establishes an efficient switch mechanism for redox control of nuclear gene activity of chloroplast antioxidants, in which Rap2.4 is a redox-sensor and a transducer of redox information. BioMed Central 2008-04-26 /pmc/articles/PMC2386467/ /pubmed/18439303 http://dx.doi.org/10.1186/1471-2229-8-48 Text en Copyright © 2008 Shaikhali et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shaikhali, Jehad
Heiber, Isabelle
Seidel, Thorsten
Ströher, Elke
Hiltscher, Heiko
Birkmann, Stefan
Dietz, Karl-Josef
Baier, Margarete
The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes
title The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes
title_full The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes
title_fullStr The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes
title_full_unstemmed The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes
title_short The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes
title_sort redox-sensitive transcription factor rap2.4a controls nuclear expression of 2-cys peroxiredoxin a and other chloroplast antioxidant enzymes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386467/
https://www.ncbi.nlm.nih.gov/pubmed/18439303
http://dx.doi.org/10.1186/1471-2229-8-48
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