Cargando…
The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice
BACKGROUND: Plants respond to extracellularly perceived abiotic stresses such as low temperature, drought, and salinity by activation of complex intracellular signaling cascades that regulate acclimatory biochemical and physiological changes. Protein kinases are major signal transduction factors tha...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386468/ https://www.ncbi.nlm.nih.gov/pubmed/18442365 http://dx.doi.org/10.1186/1471-2229-8-49 |
_version_ | 1782155238779650048 |
---|---|
author | Diédhiou, Calliste J Popova, Olga V Dietz, Karl-Josef Golldack, Dortje |
author_facet | Diédhiou, Calliste J Popova, Olga V Dietz, Karl-Josef Golldack, Dortje |
author_sort | Diédhiou, Calliste J |
collection | PubMed |
description | BACKGROUND: Plants respond to extracellularly perceived abiotic stresses such as low temperature, drought, and salinity by activation of complex intracellular signaling cascades that regulate acclimatory biochemical and physiological changes. Protein kinases are major signal transduction factors that have a central role in mediating acclimation to environmental changes in eukaryotic organisms. In this study, we characterized the function of the sucrose nonfermenting 1-related protein kinase2 (SnRK2) SAPK4 in the salt stress response of rice. RESULTS: Translational fusion of SAPK4 with the green fluorescent protein (GFP) showed subcellular localization in cytoplasm and nucleus. To examine the role of SAPK4 in salt tolerance we generated transgenic rice plants with over-expression of rice SAPK4 under control of the CaMV-35S promoter. Induced expression of SAPK4 resulted in improved germination, growth and development under salt stress both in seedlings and mature plants. In response to salt stress, the SAPK4-overexpressing rice accumulated less Na(+ )and Cl(- )and showed improved photosynthesis. SAPK4-regulated genes with functions in ion homeostasis and oxidative stress response were identified: the vacuolar H(+)-ATPase, the Na(+)/H(+ )antiporter NHX1, the Cl(- )channel OsCLC1 and a catalase. CONCLUSION: Our results show that SAPK4 regulates ion homeostasis and growth and development under salinity and suggest function of SAPK4 as a regulatory factor in plant salt stress acclimation. Identification of signaling elements involved in stress adaptation in plants presents a powerful approach to identify transcriptional activators of adaptive mechanisms to environmental changes that have the potential to improve tolerance in crop plants. |
format | Text |
id | pubmed-2386468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-23864682008-05-16 The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice Diédhiou, Calliste J Popova, Olga V Dietz, Karl-Josef Golldack, Dortje BMC Plant Biol Research Article BACKGROUND: Plants respond to extracellularly perceived abiotic stresses such as low temperature, drought, and salinity by activation of complex intracellular signaling cascades that regulate acclimatory biochemical and physiological changes. Protein kinases are major signal transduction factors that have a central role in mediating acclimation to environmental changes in eukaryotic organisms. In this study, we characterized the function of the sucrose nonfermenting 1-related protein kinase2 (SnRK2) SAPK4 in the salt stress response of rice. RESULTS: Translational fusion of SAPK4 with the green fluorescent protein (GFP) showed subcellular localization in cytoplasm and nucleus. To examine the role of SAPK4 in salt tolerance we generated transgenic rice plants with over-expression of rice SAPK4 under control of the CaMV-35S promoter. Induced expression of SAPK4 resulted in improved germination, growth and development under salt stress both in seedlings and mature plants. In response to salt stress, the SAPK4-overexpressing rice accumulated less Na(+ )and Cl(- )and showed improved photosynthesis. SAPK4-regulated genes with functions in ion homeostasis and oxidative stress response were identified: the vacuolar H(+)-ATPase, the Na(+)/H(+ )antiporter NHX1, the Cl(- )channel OsCLC1 and a catalase. CONCLUSION: Our results show that SAPK4 regulates ion homeostasis and growth and development under salinity and suggest function of SAPK4 as a regulatory factor in plant salt stress acclimation. Identification of signaling elements involved in stress adaptation in plants presents a powerful approach to identify transcriptional activators of adaptive mechanisms to environmental changes that have the potential to improve tolerance in crop plants. BioMed Central 2008-04-28 /pmc/articles/PMC2386468/ /pubmed/18442365 http://dx.doi.org/10.1186/1471-2229-8-49 Text en Copyright © 2008 Diédhiou et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Diédhiou, Calliste J Popova, Olga V Dietz, Karl-Josef Golldack, Dortje The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice |
title | The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice |
title_full | The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice |
title_fullStr | The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice |
title_full_unstemmed | The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice |
title_short | The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice |
title_sort | snf1-type serine-threonine protein kinase sapk4 regulates stress-responsive gene expression in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386468/ https://www.ncbi.nlm.nih.gov/pubmed/18442365 http://dx.doi.org/10.1186/1471-2229-8-49 |
work_keys_str_mv | AT diedhioucallistej thesnf1typeserinethreonineproteinkinasesapk4regulatesstressresponsivegeneexpressioninrice AT popovaolgav thesnf1typeserinethreonineproteinkinasesapk4regulatesstressresponsivegeneexpressioninrice AT dietzkarljosef thesnf1typeserinethreonineproteinkinasesapk4regulatesstressresponsivegeneexpressioninrice AT golldackdortje thesnf1typeserinethreonineproteinkinasesapk4regulatesstressresponsivegeneexpressioninrice AT diedhioucallistej snf1typeserinethreonineproteinkinasesapk4regulatesstressresponsivegeneexpressioninrice AT popovaolgav snf1typeserinethreonineproteinkinasesapk4regulatesstressresponsivegeneexpressioninrice AT dietzkarljosef snf1typeserinethreonineproteinkinasesapk4regulatesstressresponsivegeneexpressioninrice AT golldackdortje snf1typeserinethreonineproteinkinasesapk4regulatesstressresponsivegeneexpressioninrice |