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Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry

BACKGROUND: Fitting four-parameter sigmoidal models is one of the methods established in the analysis of quantitative real-time PCR (qPCR) data. We had observed that these models are not optimal in the fitting outcome due to the inherent constraint of symmetry around the point of inflection. Thus, w...

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Detalles Bibliográficos
Autores principales:	Spiess, Andrej-Nikolai, Feig, Caroline, Ritz, Christian
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2008
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386824/ https://www.ncbi.nlm.nih.gov/pubmed/18445269 http://dx.doi.org/10.1186/1471-2105-9-221

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