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MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer
Alterations in epithelial mucin expression are associated with carcinogenesis, but there are few data in biliary tract cancer (BTC). In pancreatic malignancy, MUC4 is a diagnostic and prognostic tumour marker, whereas MUC5AC has been proposed as a sensitive serological marker for BTC. We assessed MU...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2391120/ https://www.ncbi.nlm.nih.gov/pubmed/18475301 http://dx.doi.org/10.1038/sj.bjc.6604364 |
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author | Matull, W R Andreola, F Loh, A Adiguzel, Z Deheragoda, M Qureshi, U Batra, S K Swallow, D M Pereira, S P |
author_facet | Matull, W R Andreola, F Loh, A Adiguzel, Z Deheragoda, M Qureshi, U Batra, S K Swallow, D M Pereira, S P |
author_sort | Matull, W R |
collection | PubMed |
description | Alterations in epithelial mucin expression are associated with carcinogenesis, but there are few data in biliary tract cancer (BTC). In pancreatic malignancy, MUC4 is a diagnostic and prognostic tumour marker, whereas MUC5AC has been proposed as a sensitive serological marker for BTC. We assessed MUC4 and MUC5AC expression in (i) prospectively collected bile and serum specimens from 72 patients with biliary obstruction (39 BTC) by real-time reverse transcriptase–PCR (qPCR) and western blot analysis, and (ii) 79 archived biliary tissues (69 BTC) by immunohistochemistry. In bile, MUC4 protein was detected in 27% of BTC and 29% of primary sclerosing cholangitis (PSC) cases, but not in other benign and malignant biliary diseases (P<0.01 and P=0.06). qPCR revealed a 1.9-fold increased MUC4 mRNA expression in BTC patients’ bile compared with benign disease. In archived tissues, MUC4 protein was detected in 37% of BTC but in none of the benign samples (P=0.03). In serum, MUC5AC was found exclusively in BTC and PSC sera (44% and 13%, respectively; P<0.001 for BTC vs non-BTC) and correlated negatively with BTC survival. Biliary MUC4 and serum MUC5AC are highly specific tumour-associated mucins that may be useful in the diagnosis and formulation of therapeutic strategies in BTC. |
format | Text |
id | pubmed-2391120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23911202009-09-10 MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer Matull, W R Andreola, F Loh, A Adiguzel, Z Deheragoda, M Qureshi, U Batra, S K Swallow, D M Pereira, S P Br J Cancer Molecular Diagnostics Alterations in epithelial mucin expression are associated with carcinogenesis, but there are few data in biliary tract cancer (BTC). In pancreatic malignancy, MUC4 is a diagnostic and prognostic tumour marker, whereas MUC5AC has been proposed as a sensitive serological marker for BTC. We assessed MUC4 and MUC5AC expression in (i) prospectively collected bile and serum specimens from 72 patients with biliary obstruction (39 BTC) by real-time reverse transcriptase–PCR (qPCR) and western blot analysis, and (ii) 79 archived biliary tissues (69 BTC) by immunohistochemistry. In bile, MUC4 protein was detected in 27% of BTC and 29% of primary sclerosing cholangitis (PSC) cases, but not in other benign and malignant biliary diseases (P<0.01 and P=0.06). qPCR revealed a 1.9-fold increased MUC4 mRNA expression in BTC patients’ bile compared with benign disease. In archived tissues, MUC4 protein was detected in 37% of BTC but in none of the benign samples (P=0.03). In serum, MUC5AC was found exclusively in BTC and PSC sera (44% and 13%, respectively; P<0.001 for BTC vs non-BTC) and correlated negatively with BTC survival. Biliary MUC4 and serum MUC5AC are highly specific tumour-associated mucins that may be useful in the diagnosis and formulation of therapeutic strategies in BTC. Nature Publishing Group 2008-05-20 2008-05-13 /pmc/articles/PMC2391120/ /pubmed/18475301 http://dx.doi.org/10.1038/sj.bjc.6604364 Text en Copyright © 2008 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Molecular Diagnostics Matull, W R Andreola, F Loh, A Adiguzel, Z Deheragoda, M Qureshi, U Batra, S K Swallow, D M Pereira, S P MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer |
title | MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer |
title_full | MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer |
title_fullStr | MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer |
title_full_unstemmed | MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer |
title_short | MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer |
title_sort | muc4 and muc5ac are highly specific tumour-associated mucins in biliary tract cancer |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2391120/ https://www.ncbi.nlm.nih.gov/pubmed/18475301 http://dx.doi.org/10.1038/sj.bjc.6604364 |
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