High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81

The study of membrane protein structure and function requires their high-level expression and purification in fully functional form. We previously used a tetracycline-inducible stable mammalian cell line, HEK293S-TetR, for regulated high-level expression of G-protein coupled receptors. We here repor...

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Bibliographic Details
Main Authors: Takayama, Hidehito, Chelikani, Prashen, Reeves, Philip J., Zhang, Shuguang, Khorana, H. Gobind
Format: Text
Language:English
Published: Public Library of Science 2008
Subjects:
Research Article
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2391292/
https://www.ncbi.nlm.nih.gov/pubmed/18523555
http://dx.doi.org/10.1371/journal.pone.0002314

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2391292/
https://www.ncbi.nlm.nih.gov/pubmed/18523555
http://dx.doi.org/10.1371/journal.pone.0002314

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