Expression of endogenous granzyme B in a subset of human primary breast carcinomas

Granzyme B (GrB) is the prototypic member of a serine protease family primarily used by cytotoxic lymphocytes to kill target cells. We report here that, by immunohistochemical staining of paraffin-embedded tumour sections, GrB protein was unexpectedly detected in malignant cells of a subset of breas...

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Detalles Bibliográficos
Autores principales: Hu, S X, Wang, S, Wang, J P, Mills, G B, Zhou, Y, Xu, H-J
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2003
Materias:
Molecular and Cellular Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2394226/
https://www.ncbi.nlm.nih.gov/pubmed/12838314
http://dx.doi.org/10.1038/sj.bjc.6601051
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author Hu, S X
Wang, S
Wang, J P
Mills, G B
Zhou, Y
Xu, H-J
author_facet Hu, S X
Wang, S
Wang, J P
Mills, G B
Zhou, Y
Xu, H-J
author_sort Hu, S X
collection PubMed
description Granzyme B (GrB) is the prototypic member of a serine protease family primarily used by cytotoxic lymphocytes to kill target cells. We report here that, by immunohistochemical staining of paraffin-embedded tumour sections, GrB protein was unexpectedly detected in malignant cells of a subset of breast cancers and their adjacent reactive endothelial and mesenchymal cells in which endogenous retinoblastoma protein (pRB) is overexpressed. The identity of the endogenous GrB was further confirmed experimentally in RB-deficient breast carcinoma cell culture upon overexpression of ectopic pRB. Our finding extends the recent paradigm-shifting trend for a more diverse biological role of granzyme B, and might provide a rational basis for exploring its potential prognostic value in a variety of human cancers.
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spelling pubmed-23942262009-09-10 Expression of endogenous granzyme B in a subset of human primary breast carcinomas Hu, S X Wang, S Wang, J P Mills, G B Zhou, Y Xu, H-J Br J Cancer Molecular and Cellular Pathology Granzyme B (GrB) is the prototypic member of a serine protease family primarily used by cytotoxic lymphocytes to kill target cells. We report here that, by immunohistochemical staining of paraffin-embedded tumour sections, GrB protein was unexpectedly detected in malignant cells of a subset of breast cancers and their adjacent reactive endothelial and mesenchymal cells in which endogenous retinoblastoma protein (pRB) is overexpressed. The identity of the endogenous GrB was further confirmed experimentally in RB-deficient breast carcinoma cell culture upon overexpression of ectopic pRB. Our finding extends the recent paradigm-shifting trend for a more diverse biological role of granzyme B, and might provide a rational basis for exploring its potential prognostic value in a variety of human cancers. Nature Publishing Group 2003-07-07 2003-07-01 /pmc/articles/PMC2394226/ /pubmed/12838314 http://dx.doi.org/10.1038/sj.bjc.6601051 Text en Copyright © 2003 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular and Cellular Pathology
Hu, S X
Wang, S
Wang, J P
Mills, G B
Zhou, Y
Xu, H-J
Expression of endogenous granzyme B in a subset of human primary breast carcinomas
title Expression of endogenous granzyme B in a subset of human primary breast carcinomas
title_full Expression of endogenous granzyme B in a subset of human primary breast carcinomas
title_fullStr Expression of endogenous granzyme B in a subset of human primary breast carcinomas
title_full_unstemmed Expression of endogenous granzyme B in a subset of human primary breast carcinomas
title_short Expression of endogenous granzyme B in a subset of human primary breast carcinomas
title_sort expression of endogenous granzyme b in a subset of human primary breast carcinomas
topic Molecular and Cellular Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2394226/
https://www.ncbi.nlm.nih.gov/pubmed/12838314
http://dx.doi.org/10.1038/sj.bjc.6601051
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