RAD marker microarrays enable rapid mapping of zebrafish mutations

We constructed a restriction site associated DNA (RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, w...

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Detalles Bibliográficos
Autores principales: Miller, Michael R, Atwood, Tressa S, Eames, B Frank, Eberhart, Johann K, Yan, Yi-Lin, Postlethwait, John H, Johnson, Eric A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2394753/
https://www.ncbi.nlm.nih.gov/pubmed/17553171
http://dx.doi.org/10.1186/gb-2007-8-6-r105
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author Miller, Michael R
Atwood, Tressa S
Eames, B Frank
Eberhart, Johann K
Yan, Yi-Lin
Postlethwait, John H
Johnson, Eric A
author_facet Miller, Michael R
Atwood, Tressa S
Eames, B Frank
Eberhart, Johann K
Yan, Yi-Lin
Postlethwait, John H
Johnson, Eric A
author_sort Miller, Michael R
collection PubMed
description We constructed a restriction site associated DNA (RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.
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spelling pubmed-23947532008-05-24 RAD marker microarrays enable rapid mapping of zebrafish mutations Miller, Michael R Atwood, Tressa S Eames, B Frank Eberhart, Johann K Yan, Yi-Lin Postlethwait, John H Johnson, Eric A Genome Biol Method We constructed a restriction site associated DNA (RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms. BioMed Central 2007 2007-06-06 /pmc/articles/PMC2394753/ /pubmed/17553171 http://dx.doi.org/10.1186/gb-2007-8-6-r105 Text en Copyright © 2007 Miller et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Miller, Michael R
Atwood, Tressa S
Eames, B Frank
Eberhart, Johann K
Yan, Yi-Lin
Postlethwait, John H
Johnson, Eric A
RAD marker microarrays enable rapid mapping of zebrafish mutations
title RAD marker microarrays enable rapid mapping of zebrafish mutations
title_full RAD marker microarrays enable rapid mapping of zebrafish mutations
title_fullStr RAD marker microarrays enable rapid mapping of zebrafish mutations
title_full_unstemmed RAD marker microarrays enable rapid mapping of zebrafish mutations
title_short RAD marker microarrays enable rapid mapping of zebrafish mutations
title_sort rad marker microarrays enable rapid mapping of zebrafish mutations
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2394753/
https://www.ncbi.nlm.nih.gov/pubmed/17553171
http://dx.doi.org/10.1186/gb-2007-8-6-r105
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