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High-resolution X-ray microdiffraction analysis of natural teeth
The main component of natural teeth was determined many years ago as calcium phosphate, mostly in the form of hydroxyapatite with different crystallites. In the past, the method used in tooth crystal investigation has been mainly powder X-ray diffraction analysis, but this method has its drawbacks,...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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International Union of Crystallography
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2394821/ https://www.ncbi.nlm.nih.gov/pubmed/18421147 http://dx.doi.org/10.1107/S0909049508003397 |
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author | Xue, Jing Zhang, Linling Zou, Ling Liao, Yunmao Li, Jiyao Xiao, Liying Li, Wei |
author_facet | Xue, Jing Zhang, Linling Zou, Ling Liao, Yunmao Li, Jiyao Xiao, Liying Li, Wei |
author_sort | Xue, Jing |
collection | PubMed |
description | The main component of natural teeth was determined many years ago as calcium phosphate, mostly in the form of hydroxyapatite with different crystallites. In the past, the method used in tooth crystal investigation has been mainly powder X-ray diffraction analysis, but this method has its drawbacks, i.e. the destruction of the natural tooth structure and the difficulty in examining the preferred orientation in different layers of the tooth. During the last century, microzone X-ray diffraction on the tooth surface was carried out, but, as the technology was less sophisticated, the results obtained were not very detailed. The newly developed microdiffraction equipment permits analysis of the microzone of teeth in situ. To test this new microdiffraction equipment, microdiffraction analysis of one natural healthy deciduous molar tooth and one carious deciduous molar tooth has been performed, using a Bruker D8 instrument. Phase analysis of the two teeth was performed; the crystal size at six test points in the natural healthy tooth was calculated by reflection (211), and the crystal preferred orientation of reflection (300) and reflection (002) at six test points in the natural healthy tooth were compared. The results showed that the tooth was a kind of biological mixed crystal composed of several crystal phases, the main crystal phase being hydroxyapatite. The crystal size grew larger going from the dentin to the enamel. The crystal preferred orientation mainly existed in the enamel, especially in the reflection (002). From our experiment, layer orientation and continuous crystal variations in teeth could be conveniently studied using fast online measurements by high-resolution X-ray microdiffraction equipment. |
format | Text |
id | pubmed-2394821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-23948212009-03-05 High-resolution X-ray microdiffraction analysis of natural teeth Xue, Jing Zhang, Linling Zou, Ling Liao, Yunmao Li, Jiyao Xiao, Liying Li, Wei J Synchrotron Radiat Diffraction Structural Biology The main component of natural teeth was determined many years ago as calcium phosphate, mostly in the form of hydroxyapatite with different crystallites. In the past, the method used in tooth crystal investigation has been mainly powder X-ray diffraction analysis, but this method has its drawbacks, i.e. the destruction of the natural tooth structure and the difficulty in examining the preferred orientation in different layers of the tooth. During the last century, microzone X-ray diffraction on the tooth surface was carried out, but, as the technology was less sophisticated, the results obtained were not very detailed. The newly developed microdiffraction equipment permits analysis of the microzone of teeth in situ. To test this new microdiffraction equipment, microdiffraction analysis of one natural healthy deciduous molar tooth and one carious deciduous molar tooth has been performed, using a Bruker D8 instrument. Phase analysis of the two teeth was performed; the crystal size at six test points in the natural healthy tooth was calculated by reflection (211), and the crystal preferred orientation of reflection (300) and reflection (002) at six test points in the natural healthy tooth were compared. The results showed that the tooth was a kind of biological mixed crystal composed of several crystal phases, the main crystal phase being hydroxyapatite. The crystal size grew larger going from the dentin to the enamel. The crystal preferred orientation mainly existed in the enamel, especially in the reflection (002). From our experiment, layer orientation and continuous crystal variations in teeth could be conveniently studied using fast online measurements by high-resolution X-ray microdiffraction equipment. International Union of Crystallography 2008-05-01 2008-04-18 /pmc/articles/PMC2394821/ /pubmed/18421147 http://dx.doi.org/10.1107/S0909049508003397 Text en © International Union of Crystallography 2008 http://journals.iucr.org/services/termsofuse.html This is an open-access article distributed under the terms described at http://journals.iucr.org/services/termsofuse.html. |
spellingShingle | Diffraction Structural Biology Xue, Jing Zhang, Linling Zou, Ling Liao, Yunmao Li, Jiyao Xiao, Liying Li, Wei High-resolution X-ray microdiffraction analysis of natural teeth |
title | High-resolution X-ray microdiffraction analysis of natural teeth |
title_full | High-resolution X-ray microdiffraction analysis of natural teeth |
title_fullStr | High-resolution X-ray microdiffraction analysis of natural teeth |
title_full_unstemmed | High-resolution X-ray microdiffraction analysis of natural teeth |
title_short | High-resolution X-ray microdiffraction analysis of natural teeth |
title_sort | high-resolution x-ray microdiffraction analysis of natural teeth |
topic | Diffraction Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2394821/ https://www.ncbi.nlm.nih.gov/pubmed/18421147 http://dx.doi.org/10.1107/S0909049508003397 |
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