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Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data
With the emergence of genome-wide colorimetric in situ hybridization (ISH) data sets such as the Allen Brain Atlas, it is important to understand the relationship between this gene expression modality and those derived from more quantitative based technologies. This study introduces a novel method f...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395252/ https://www.ncbi.nlm.nih.gov/pubmed/18234097 http://dx.doi.org/10.1186/gb-2008-9-1-r23 |
| _version_ | 1782155463480049664 |
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| author | Lee, Chang-Kyu Sunkin, Susan M Kuan, Chihchau Thompson, Carol L Pathak, Sayan Ng, Lydia Lau, Chris Fischer, Shanna Mortrud, Marty Slaughterbeck, Cliff Jones, Allan Lein, Ed Hawrylycz, Michael |
| author_facet | Lee, Chang-Kyu Sunkin, Susan M Kuan, Chihchau Thompson, Carol L Pathak, Sayan Ng, Lydia Lau, Chris Fischer, Shanna Mortrud, Marty Slaughterbeck, Cliff Jones, Allan Lein, Ed Hawrylycz, Michael |
| author_sort | Lee, Chang-Kyu |
| collection | PubMed |
| description | With the emergence of genome-wide colorimetric in situ hybridization (ISH) data sets such as the Allen Brain Atlas, it is important to understand the relationship between this gene expression modality and those derived from more quantitative based technologies. This study introduces a novel method for standardized relative quantification of colorimetric ISH signal that enables a large-scale cross-platform expression level comparison of ISH with two publicly available microarray brain data sources. |
| format | Text |
| id | pubmed-2395252 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2008 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-23952522008-05-29 Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data Lee, Chang-Kyu Sunkin, Susan M Kuan, Chihchau Thompson, Carol L Pathak, Sayan Ng, Lydia Lau, Chris Fischer, Shanna Mortrud, Marty Slaughterbeck, Cliff Jones, Allan Lein, Ed Hawrylycz, Michael Genome Biol Method With the emergence of genome-wide colorimetric in situ hybridization (ISH) data sets such as the Allen Brain Atlas, it is important to understand the relationship between this gene expression modality and those derived from more quantitative based technologies. This study introduces a novel method for standardized relative quantification of colorimetric ISH signal that enables a large-scale cross-platform expression level comparison of ISH with two publicly available microarray brain data sources. BioMed Central 2008-01-30 /pmc/articles/PMC2395252/ /pubmed/18234097 http://dx.doi.org/10.1186/gb-2008-9-1-r23 Text en Copyright © 2008 Lee et al.; licensee BioMed Central Ltd. https://creativecommons.org/licenses/by/2.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0 (https://creativecommons.org/licenses/by/2.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Method Lee, Chang-Kyu Sunkin, Susan M Kuan, Chihchau Thompson, Carol L Pathak, Sayan Ng, Lydia Lau, Chris Fischer, Shanna Mortrud, Marty Slaughterbeck, Cliff Jones, Allan Lein, Ed Hawrylycz, Michael Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data |
| title | Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data |
| title_full | Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data |
| title_fullStr | Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data |
| title_full_unstemmed | Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data |
| title_short | Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data |
| title_sort | quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data |
| topic | Method |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395252/ https://www.ncbi.nlm.nih.gov/pubmed/18234097 http://dx.doi.org/10.1186/gb-2008-9-1-r23 |
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