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Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity
BACKGROUND: Investigations performed in mice and humans have acknowledged obesity as a low-grade inflammatory disease. Several molecular mechanisms have been convincingly shown to be involved in activating inflammatory processes and altering cell composition in white adipose tissue (WAT). However, t...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395253/ https://www.ncbi.nlm.nih.gov/pubmed/18208606 http://dx.doi.org/10.1186/gb-2008-9-1-r14 |
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author | Henegar, Corneliu Tordjman, Joan Achard, Vincent Lacasa, Danièle Cremer, Isabelle Guerre-Millo, Michèle Poitou, Christine Basdevant, Arnaud Stich, Vladimir Viguerie, Nathalie Langin, Dominique Bedossa, Pierre Zucker, Jean-Daniel Clement, Karine |
author_facet | Henegar, Corneliu Tordjman, Joan Achard, Vincent Lacasa, Danièle Cremer, Isabelle Guerre-Millo, Michèle Poitou, Christine Basdevant, Arnaud Stich, Vladimir Viguerie, Nathalie Langin, Dominique Bedossa, Pierre Zucker, Jean-Daniel Clement, Karine |
author_sort | Henegar, Corneliu |
collection | PubMed |
description | BACKGROUND: Investigations performed in mice and humans have acknowledged obesity as a low-grade inflammatory disease. Several molecular mechanisms have been convincingly shown to be involved in activating inflammatory processes and altering cell composition in white adipose tissue (WAT). However, the overall importance of these alterations, and their long-term impact on the metabolic functions of the WAT and on its morphology, remain unclear. RESULTS: Here, we analyzed the transcriptomic signature of the subcutaneous WAT in obese human subjects, in stable weight conditions and after weight loss following bariatric surgery. An original integrative functional genomics approach was applied to quantify relations between relevant structural and functional themes annotating differentially expressed genes in order to construct a comprehensive map of transcriptional interactions defining the obese WAT. These analyses highlighted a significant up-regulation of genes and biological themes related to extracellular matrix (ECM) constituents, including members of the integrin family, and suggested that these elements could play a major mediating role in a chain of interactions that connect local inflammatory phenomena to the alteration of WAT metabolic functions in obese subjects. Tissue and cellular investigations, driven by the analysis of transcriptional interactions, revealed an increased amount of interstitial fibrosis in obese WAT, associated with an infiltration of different types of inflammatory cells, and suggest that phenotypic alterations of human pre-adipocytes, induced by a pro-inflammatory environment, may lead to an excessive synthesis of ECM components. CONCLUSION: This study opens new perspectives in understanding the biology of human WAT and its pathologic changes indicative of tissue deterioration associated with the development of obesity. |
format | Text |
id | pubmed-2395253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-23952532008-05-24 Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity Henegar, Corneliu Tordjman, Joan Achard, Vincent Lacasa, Danièle Cremer, Isabelle Guerre-Millo, Michèle Poitou, Christine Basdevant, Arnaud Stich, Vladimir Viguerie, Nathalie Langin, Dominique Bedossa, Pierre Zucker, Jean-Daniel Clement, Karine Genome Biol Research BACKGROUND: Investigations performed in mice and humans have acknowledged obesity as a low-grade inflammatory disease. Several molecular mechanisms have been convincingly shown to be involved in activating inflammatory processes and altering cell composition in white adipose tissue (WAT). However, the overall importance of these alterations, and their long-term impact on the metabolic functions of the WAT and on its morphology, remain unclear. RESULTS: Here, we analyzed the transcriptomic signature of the subcutaneous WAT in obese human subjects, in stable weight conditions and after weight loss following bariatric surgery. An original integrative functional genomics approach was applied to quantify relations between relevant structural and functional themes annotating differentially expressed genes in order to construct a comprehensive map of transcriptional interactions defining the obese WAT. These analyses highlighted a significant up-regulation of genes and biological themes related to extracellular matrix (ECM) constituents, including members of the integrin family, and suggested that these elements could play a major mediating role in a chain of interactions that connect local inflammatory phenomena to the alteration of WAT metabolic functions in obese subjects. Tissue and cellular investigations, driven by the analysis of transcriptional interactions, revealed an increased amount of interstitial fibrosis in obese WAT, associated with an infiltration of different types of inflammatory cells, and suggest that phenotypic alterations of human pre-adipocytes, induced by a pro-inflammatory environment, may lead to an excessive synthesis of ECM components. CONCLUSION: This study opens new perspectives in understanding the biology of human WAT and its pathologic changes indicative of tissue deterioration associated with the development of obesity. BioMed Central 2008-01-21 /pmc/articles/PMC2395253/ /pubmed/18208606 http://dx.doi.org/10.1186/gb-2008-9-1-r14 Text en Copyright © 2008 Henegar et al.; licensee BioMed Central Ltd. https://creativecommons.org/licenses/by/2.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0 (https://creativecommons.org/licenses/by/2.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Henegar, Corneliu Tordjman, Joan Achard, Vincent Lacasa, Danièle Cremer, Isabelle Guerre-Millo, Michèle Poitou, Christine Basdevant, Arnaud Stich, Vladimir Viguerie, Nathalie Langin, Dominique Bedossa, Pierre Zucker, Jean-Daniel Clement, Karine Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity |
title | Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity |
title_full | Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity |
title_fullStr | Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity |
title_full_unstemmed | Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity |
title_short | Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity |
title_sort | adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395253/ https://www.ncbi.nlm.nih.gov/pubmed/18208606 http://dx.doi.org/10.1186/gb-2008-9-1-r14 |
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