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Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice
In order to develop preclinical models of malignant astrocytomas and oligodendrogliomas, a series of 54 resected gliomas (37 from oligodendroglial lineage and 17 from astrocytic lineage) were xenografted subcutaneously into nude mice. Molecular alterations commonly observed in gliomas subtypes, incl...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2003
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395278/ https://www.ncbi.nlm.nih.gov/pubmed/14676814 http://dx.doi.org/10.1038/sj.bjc.6601466 |
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author | Leuraud, P Taillandier, L Aguirre-Cruz, L Medioni, J Crinière, E Marie, Y Dutrillaux, A M Kujas, M Duprez, A Delattre, J-Y Poupon, M-F Sanson, M |
author_facet | Leuraud, P Taillandier, L Aguirre-Cruz, L Medioni, J Crinière, E Marie, Y Dutrillaux, A M Kujas, M Duprez, A Delattre, J-Y Poupon, M-F Sanson, M |
author_sort | Leuraud, P |
collection | PubMed |
description | In order to develop preclinical models of malignant astrocytomas and oligodendrogliomas, a series of 54 resected gliomas (37 from oligodendroglial lineage and 17 from astrocytic lineage) were xenografted subcutaneously into nude mice. Molecular alterations commonly observed in gliomas subtypes, including LOH 1p and 1q, LOH 19q, LOH 10p and 10q, LOH 9p, TP53 and PTEN mutations, EGFR amplification, CDKN2A homozygous deletion and telomerase reactivation were systematically screened in the original and xenografted tumours. In all, 23 gliomas grew in nude mice. The most anaplastic tumours were selected as shown by pathological and molecular studies of the original tumour as well as shorter survival in patients whose tumours were successfully grafted. Comparison between the two growth profiles showed that 10q LOH and EGFR amplification gave a tumorigenic advantage. With a few exceptions, the genetic pattern was remarkably stable before and after growth in nude mice. These results suggest that subcutaneous xenografts are useful and reproducible models to analyse the molecular profile of malignant astrocytoma and oligodendroglioma. This represents the first step to improve our understanding of the correlations between molecular alterations and response to standard or experimental therapies. |
format | Text |
id | pubmed-2395278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23952782009-09-10 Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice Leuraud, P Taillandier, L Aguirre-Cruz, L Medioni, J Crinière, E Marie, Y Dutrillaux, A M Kujas, M Duprez, A Delattre, J-Y Poupon, M-F Sanson, M Br J Cancer Experimental Therapeutics In order to develop preclinical models of malignant astrocytomas and oligodendrogliomas, a series of 54 resected gliomas (37 from oligodendroglial lineage and 17 from astrocytic lineage) were xenografted subcutaneously into nude mice. Molecular alterations commonly observed in gliomas subtypes, including LOH 1p and 1q, LOH 19q, LOH 10p and 10q, LOH 9p, TP53 and PTEN mutations, EGFR amplification, CDKN2A homozygous deletion and telomerase reactivation were systematically screened in the original and xenografted tumours. In all, 23 gliomas grew in nude mice. The most anaplastic tumours were selected as shown by pathological and molecular studies of the original tumour as well as shorter survival in patients whose tumours were successfully grafted. Comparison between the two growth profiles showed that 10q LOH and EGFR amplification gave a tumorigenic advantage. With a few exceptions, the genetic pattern was remarkably stable before and after growth in nude mice. These results suggest that subcutaneous xenografts are useful and reproducible models to analyse the molecular profile of malignant astrocytoma and oligodendroglioma. This represents the first step to improve our understanding of the correlations between molecular alterations and response to standard or experimental therapies. Nature Publishing Group 2003-12-15 2003-12-09 /pmc/articles/PMC2395278/ /pubmed/14676814 http://dx.doi.org/10.1038/sj.bjc.6601466 Text en Copyright © 2003 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Experimental Therapeutics Leuraud, P Taillandier, L Aguirre-Cruz, L Medioni, J Crinière, E Marie, Y Dutrillaux, A M Kujas, M Duprez, A Delattre, J-Y Poupon, M-F Sanson, M Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice |
title | Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice |
title_full | Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice |
title_fullStr | Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice |
title_full_unstemmed | Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice |
title_short | Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice |
title_sort | correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice |
topic | Experimental Therapeutics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395278/ https://www.ncbi.nlm.nih.gov/pubmed/14676814 http://dx.doi.org/10.1038/sj.bjc.6601466 |
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