Gene expression array profile of human osteosarcoma

The aims of this pilot study were to determine whether needle and open biopsies from osteosarcoma (OS) provide sufficient quality of mRNA for cDNA array analyses to gain insights into the expression profile of OS. A total of 22 samples collected from OS were used for array analyses. A primary cell c...

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Autores principales: Leonard, P, Sharp, T, Henderson, S, Hewitt, D, Pringle, J, Sandison, A, Goodship, A, Whelan, J, Boshoff, C
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2003
Materias:
Genetics and Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395284/
https://www.ncbi.nlm.nih.gov/pubmed/14676807
http://dx.doi.org/10.1038/sj.bjc.6601389
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author Leonard, P
Sharp, T
Henderson, S
Hewitt, D
Pringle, J
Sandison, A
Goodship, A
Whelan, J
Boshoff, C
author_facet Leonard, P
Sharp, T
Henderson, S
Hewitt, D
Pringle, J
Sandison, A
Goodship, A
Whelan, J
Boshoff, C
author_sort Leonard, P
collection PubMed
description The aims of this pilot study were to determine whether needle and open biopsies from osteosarcoma (OS) provide sufficient quality of mRNA for cDNA array analyses to gain insights into the expression profile of OS. A total of 22 samples collected from OS were used for array analyses. A primary cell culture was also established from one of the OS biopsies. Total RNA was extracted and probes were generated for cDNA arrays. cDNA probes were made for all the 22 samples. Two of these samples were needle core bone biopsies. Statistical analysis confirmed the reliability of array data obtained in 16 of the 22 samples. Known genes involved in bone metabolism and osteosarcoma were identified as highly expressed, and the putative new marker Ezrin was also identified. Confirmatory immunohistochemical staining using the Ezrin antibody was performed in a selection of samples.
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spelling pubmed-23952842009-09-10 Gene expression array profile of human osteosarcoma Leonard, P Sharp, T Henderson, S Hewitt, D Pringle, J Sandison, A Goodship, A Whelan, J Boshoff, C Br J Cancer Genetics and Genomics The aims of this pilot study were to determine whether needle and open biopsies from osteosarcoma (OS) provide sufficient quality of mRNA for cDNA array analyses to gain insights into the expression profile of OS. A total of 22 samples collected from OS were used for array analyses. A primary cell culture was also established from one of the OS biopsies. Total RNA was extracted and probes were generated for cDNA arrays. cDNA probes were made for all the 22 samples. Two of these samples were needle core bone biopsies. Statistical analysis confirmed the reliability of array data obtained in 16 of the 22 samples. Known genes involved in bone metabolism and osteosarcoma were identified as highly expressed, and the putative new marker Ezrin was also identified. Confirmatory immunohistochemical staining using the Ezrin antibody was performed in a selection of samples. Nature Publishing Group 2003-12-15 2003-12-09 /pmc/articles/PMC2395284/ /pubmed/14676807 http://dx.doi.org/10.1038/sj.bjc.6601389 Text en Copyright © 2003 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Genetics and Genomics
Leonard, P
Sharp, T
Henderson, S
Hewitt, D
Pringle, J
Sandison, A
Goodship, A
Whelan, J
Boshoff, C
Gene expression array profile of human osteosarcoma
title Gene expression array profile of human osteosarcoma
title_full Gene expression array profile of human osteosarcoma
title_fullStr Gene expression array profile of human osteosarcoma
title_full_unstemmed Gene expression array profile of human osteosarcoma
title_short Gene expression array profile of human osteosarcoma
title_sort gene expression array profile of human osteosarcoma
topic Genetics and Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395284/
https://www.ncbi.nlm.nih.gov/pubmed/14676807
http://dx.doi.org/10.1038/sj.bjc.6601389
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