Cargando…

Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation

Multiple general transcription factors (GTFs), TBP and TFB, are present in many haloarchaea, and are deemed to accomplish global gene regulation. However, details and the role of GTF-directed transcriptional regulation in stress response are still not clear. Here, we report a comprehensive investiga...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Qiuhe, Han, Jing, Zhou, Ligang, Coker, James A., DasSarma, Priya, DasSarma, Shiladitya, Xiang, Hua
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396416/
https://www.ncbi.nlm.nih.gov/pubmed/18390887
http://dx.doi.org/10.1093/nar/gkn152
_version_ 1782155556823236608
author Lu, Qiuhe
Han, Jing
Zhou, Ligang
Coker, James A.
DasSarma, Priya
DasSarma, Shiladitya
Xiang, Hua
author_facet Lu, Qiuhe
Han, Jing
Zhou, Ligang
Coker, James A.
DasSarma, Priya
DasSarma, Shiladitya
Xiang, Hua
author_sort Lu, Qiuhe
collection PubMed
description Multiple general transcription factors (GTFs), TBP and TFB, are present in many haloarchaea, and are deemed to accomplish global gene regulation. However, details and the role of GTF-directed transcriptional regulation in stress response are still not clear. Here, we report a comprehensive investigation of the regulatory mechanism of a heat-induced gene (hsp5) from Halobacterium salinarum. We demonstrated by mutation analysis that the sequences 5′ and 3′ to the core elements (TATA box and BRE) of the hsp5 promoter (P(hsp)(5)) did not significantly affect the basal and heat-induced gene expression, as long as the transcription initiation site was not altered. Moreover, the BRE and TATA box of P(hsp)(5) were sufficient to render a nonheat-responsive promoter heat-inducible, in both Haloferax volcanii and Halobacterium sp. NRC-1. DNA–protein interactions revealed that two heat-inducible GTFs, TFB2 from H. volcanii and TFBb from Halobacterium sp. NRC-1, could specifically bind to P(hsp)(5) likely in a temperature-dependent manner. Taken together, the heat-responsiveness of P(hsp)(5) was mainly ascribed to the core promoter elements that were efficiently recognized by specific heat-induced GTFs at elevated temperature, thus providing a new paradigm for GTF-directed gene regulation in the domain of Archaea.
format Text
id pubmed-2396416
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-23964162008-05-28 Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation Lu, Qiuhe Han, Jing Zhou, Ligang Coker, James A. DasSarma, Priya DasSarma, Shiladitya Xiang, Hua Nucleic Acids Res Molecular Biology Multiple general transcription factors (GTFs), TBP and TFB, are present in many haloarchaea, and are deemed to accomplish global gene regulation. However, details and the role of GTF-directed transcriptional regulation in stress response are still not clear. Here, we report a comprehensive investigation of the regulatory mechanism of a heat-induced gene (hsp5) from Halobacterium salinarum. We demonstrated by mutation analysis that the sequences 5′ and 3′ to the core elements (TATA box and BRE) of the hsp5 promoter (P(hsp)(5)) did not significantly affect the basal and heat-induced gene expression, as long as the transcription initiation site was not altered. Moreover, the BRE and TATA box of P(hsp)(5) were sufficient to render a nonheat-responsive promoter heat-inducible, in both Haloferax volcanii and Halobacterium sp. NRC-1. DNA–protein interactions revealed that two heat-inducible GTFs, TFB2 from H. volcanii and TFBb from Halobacterium sp. NRC-1, could specifically bind to P(hsp)(5) likely in a temperature-dependent manner. Taken together, the heat-responsiveness of P(hsp)(5) was mainly ascribed to the core promoter elements that were efficiently recognized by specific heat-induced GTFs at elevated temperature, thus providing a new paradigm for GTF-directed gene regulation in the domain of Archaea. Oxford University Press 2008-05 2008-04-05 /pmc/articles/PMC2396416/ /pubmed/18390887 http://dx.doi.org/10.1093/nar/gkn152 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Lu, Qiuhe
Han, Jing
Zhou, Ligang
Coker, James A.
DasSarma, Priya
DasSarma, Shiladitya
Xiang, Hua
Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation
title Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation
title_full Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation
title_fullStr Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation
title_full_unstemmed Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation
title_short Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation
title_sort dissection of the regulatory mechanism of a heat-shock responsive promoter in haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396416/
https://www.ncbi.nlm.nih.gov/pubmed/18390887
http://dx.doi.org/10.1093/nar/gkn152
work_keys_str_mv AT luqiuhe dissectionoftheregulatorymechanismofaheatshockresponsivepromoterinhaloarchaeaanewparadigmforgeneraltranscriptionfactordirectedarchaealgeneregulation
AT hanjing dissectionoftheregulatorymechanismofaheatshockresponsivepromoterinhaloarchaeaanewparadigmforgeneraltranscriptionfactordirectedarchaealgeneregulation
AT zhouligang dissectionoftheregulatorymechanismofaheatshockresponsivepromoterinhaloarchaeaanewparadigmforgeneraltranscriptionfactordirectedarchaealgeneregulation
AT cokerjamesa dissectionoftheregulatorymechanismofaheatshockresponsivepromoterinhaloarchaeaanewparadigmforgeneraltranscriptionfactordirectedarchaealgeneregulation
AT dassarmapriya dissectionoftheregulatorymechanismofaheatshockresponsivepromoterinhaloarchaeaanewparadigmforgeneraltranscriptionfactordirectedarchaealgeneregulation
AT dassarmashiladitya dissectionoftheregulatorymechanismofaheatshockresponsivepromoterinhaloarchaeaanewparadigmforgeneraltranscriptionfactordirectedarchaealgeneregulation
AT xianghua dissectionoftheregulatorymechanismofaheatshockresponsivepromoterinhaloarchaeaanewparadigmforgeneraltranscriptionfactordirectedarchaealgeneregulation