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Protein Expression in the Nucleus Accumbens of Rats Exposed to Developmental Vitamin D Deficiency

INTRODUCTION: Developmental vitamin D (DVD) deficiency is a candidate risk factor for schizophrenia. Animal models have confirmed that DVD deficiency is associated with a range of altered genomic, proteomic, structural and behavioural outcomes in the rat. Because the nucleus accumbens has been impli...

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Autores principales: McGrath, John, Iwazaki, Takeshi, Eyles, Darryl, Burne, Thomas, Cui, Xiaoying, Ko, Pauline, Matsumoto, Izuru
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396486/
https://www.ncbi.nlm.nih.gov/pubmed/18545652
http://dx.doi.org/10.1371/journal.pone.0002383
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author McGrath, John
Iwazaki, Takeshi
Eyles, Darryl
Burne, Thomas
Cui, Xiaoying
Ko, Pauline
Matsumoto, Izuru
author_facet McGrath, John
Iwazaki, Takeshi
Eyles, Darryl
Burne, Thomas
Cui, Xiaoying
Ko, Pauline
Matsumoto, Izuru
author_sort McGrath, John
collection PubMed
description INTRODUCTION: Developmental vitamin D (DVD) deficiency is a candidate risk factor for schizophrenia. Animal models have confirmed that DVD deficiency is associated with a range of altered genomic, proteomic, structural and behavioural outcomes in the rat. Because the nucleus accumbens has been implicated in neuropsychiatric disorders, in the current study we examined protein expression in this region in adult rats exposed to DVD deficiency METHODS: Female Sprague Dawley rats were maintained on a vitamin D deficient diet for 6 weeks, mated and allowed to give birth, after which a diet containing vitamin D was reintroduced. Male adult offspring (n = 8) were compared to control male (n = 8). 2-D gel electrophoresis-based proteomics and mass spectroscopy were used to investigate differential protein expression. RESULTS: There were 35 spots, mapped to 33 unique proteins, which were significantly different between the two groups. Of these, 22 were down-regulated and 13 up-regulated. The fold changes were uniformly small, with the largest FC being −1.67. Within the significantly different spots, three calcium binding proteins (calbindin1, calbindin2 and hippocalcin) were altered. Other proteins associated with DVD deficiency related to mitochondrial function, and the dynamin-like proteins. CONCLUSIONS: Developmental vitamin D deficiency was associated with subtle changes in protein expression in the nucleus accumbens. Disruptions in pathways related to calcium-binding proteins and mitochondrial function may underlie some of the behavioural features associated with animal models of developmental vitamin D deficiency
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spelling pubmed-23964862008-06-11 Protein Expression in the Nucleus Accumbens of Rats Exposed to Developmental Vitamin D Deficiency McGrath, John Iwazaki, Takeshi Eyles, Darryl Burne, Thomas Cui, Xiaoying Ko, Pauline Matsumoto, Izuru PLoS One Research Article INTRODUCTION: Developmental vitamin D (DVD) deficiency is a candidate risk factor for schizophrenia. Animal models have confirmed that DVD deficiency is associated with a range of altered genomic, proteomic, structural and behavioural outcomes in the rat. Because the nucleus accumbens has been implicated in neuropsychiatric disorders, in the current study we examined protein expression in this region in adult rats exposed to DVD deficiency METHODS: Female Sprague Dawley rats were maintained on a vitamin D deficient diet for 6 weeks, mated and allowed to give birth, after which a diet containing vitamin D was reintroduced. Male adult offspring (n = 8) were compared to control male (n = 8). 2-D gel electrophoresis-based proteomics and mass spectroscopy were used to investigate differential protein expression. RESULTS: There were 35 spots, mapped to 33 unique proteins, which were significantly different between the two groups. Of these, 22 were down-regulated and 13 up-regulated. The fold changes were uniformly small, with the largest FC being −1.67. Within the significantly different spots, three calcium binding proteins (calbindin1, calbindin2 and hippocalcin) were altered. Other proteins associated with DVD deficiency related to mitochondrial function, and the dynamin-like proteins. CONCLUSIONS: Developmental vitamin D deficiency was associated with subtle changes in protein expression in the nucleus accumbens. Disruptions in pathways related to calcium-binding proteins and mitochondrial function may underlie some of the behavioural features associated with animal models of developmental vitamin D deficiency Public Library of Science 2008-06-11 /pmc/articles/PMC2396486/ /pubmed/18545652 http://dx.doi.org/10.1371/journal.pone.0002383 Text en Mc Grath et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
McGrath, John
Iwazaki, Takeshi
Eyles, Darryl
Burne, Thomas
Cui, Xiaoying
Ko, Pauline
Matsumoto, Izuru
Protein Expression in the Nucleus Accumbens of Rats Exposed to Developmental Vitamin D Deficiency
title Protein Expression in the Nucleus Accumbens of Rats Exposed to Developmental Vitamin D Deficiency
title_full Protein Expression in the Nucleus Accumbens of Rats Exposed to Developmental Vitamin D Deficiency
title_fullStr Protein Expression in the Nucleus Accumbens of Rats Exposed to Developmental Vitamin D Deficiency
title_full_unstemmed Protein Expression in the Nucleus Accumbens of Rats Exposed to Developmental Vitamin D Deficiency
title_short Protein Expression in the Nucleus Accumbens of Rats Exposed to Developmental Vitamin D Deficiency
title_sort protein expression in the nucleus accumbens of rats exposed to developmental vitamin d deficiency
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396486/
https://www.ncbi.nlm.nih.gov/pubmed/18545652
http://dx.doi.org/10.1371/journal.pone.0002383
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