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Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle

During cytokinesis, cleavage furrow invagination requires an actomyosin-based contractile ring and addition of new membrane. Little is known about how this actin and membrane traffic to the cleavage furrow. We address this through live analysis of fluorescently tagged vesicles in postcellularized Dr...

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Detalles Bibliográficos
Autores principales: Albertson, Roger, Cao, Jian, Hsieh, Tao-shih, Sullivan, William
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396810/
https://www.ncbi.nlm.nih.gov/pubmed/18504302
http://dx.doi.org/10.1083/jcb.200803096
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author Albertson, Roger
Cao, Jian
Hsieh, Tao-shih
Sullivan, William
author_facet Albertson, Roger
Cao, Jian
Hsieh, Tao-shih
Sullivan, William
author_sort Albertson, Roger
collection PubMed
description During cytokinesis, cleavage furrow invagination requires an actomyosin-based contractile ring and addition of new membrane. Little is known about how this actin and membrane traffic to the cleavage furrow. We address this through live analysis of fluorescently tagged vesicles in postcellularized Drosophila melanogaster embryos. We find that during cytokinesis, F-actin and membrane are targeted as a unit to invaginating furrows through formation of F-actin–associated vesicles. F-actin puncta strongly colocalize with endosomal, but not Golgi-derived, vesicles. These vesicles are recruited to the cleavage furrow along the central spindle and a distinct population of microtubules (MTs) in contact with the leading furrow edge (furrow MTs). We find that Rho-specific guanine nucleotide exchange factor mutants, pebble (pbl), severely disrupt this F-actin–associated vesicle transport. These transport defects are a consequence of the pbl mutants' inability to properly form furrow MTs and the central spindle. Transport of F-actin–associated vesicles on furrow MTs and the central spindle is thus an important mechanism by which actin and membrane are delivered to the cleavage furrow.
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spelling pubmed-23968102008-12-02 Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle Albertson, Roger Cao, Jian Hsieh, Tao-shih Sullivan, William J Cell Biol Research Articles During cytokinesis, cleavage furrow invagination requires an actomyosin-based contractile ring and addition of new membrane. Little is known about how this actin and membrane traffic to the cleavage furrow. We address this through live analysis of fluorescently tagged vesicles in postcellularized Drosophila melanogaster embryos. We find that during cytokinesis, F-actin and membrane are targeted as a unit to invaginating furrows through formation of F-actin–associated vesicles. F-actin puncta strongly colocalize with endosomal, but not Golgi-derived, vesicles. These vesicles are recruited to the cleavage furrow along the central spindle and a distinct population of microtubules (MTs) in contact with the leading furrow edge (furrow MTs). We find that Rho-specific guanine nucleotide exchange factor mutants, pebble (pbl), severely disrupt this F-actin–associated vesicle transport. These transport defects are a consequence of the pbl mutants' inability to properly form furrow MTs and the central spindle. Transport of F-actin–associated vesicles on furrow MTs and the central spindle is thus an important mechanism by which actin and membrane are delivered to the cleavage furrow. The Rockefeller University Press 2008-06-02 /pmc/articles/PMC2396810/ /pubmed/18504302 http://dx.doi.org/10.1083/jcb.200803096 Text en © 2008 Albertson et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Albertson, Roger
Cao, Jian
Hsieh, Tao-shih
Sullivan, William
Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle
title Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle
title_full Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle
title_fullStr Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle
title_full_unstemmed Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle
title_short Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle
title_sort vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396810/
https://www.ncbi.nlm.nih.gov/pubmed/18504302
http://dx.doi.org/10.1083/jcb.200803096
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