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Multifactorial experimental design and the transitivity of ratios with spotted DNA microarrays
BACKGROUND: Multifactorial experimental designs using DNA microarrays are becoming increasingly common, but the extent of the transitivity of cDNA microarray expression measurements across multiple samples has yet to be explored. RESULTS: A strong correlation between direct and transitive inference...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2003
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC239860/ https://www.ncbi.nlm.nih.gov/pubmed/14525623 http://dx.doi.org/10.1186/1471-2164-4-41 |
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author | Townsend, Jeffrey P |
author_facet | Townsend, Jeffrey P |
author_sort | Townsend, Jeffrey P |
collection | PubMed |
description | BACKGROUND: Multifactorial experimental designs using DNA microarrays are becoming increasingly common, but the extent of the transitivity of cDNA microarray expression measurements across multiple samples has yet to be explored. RESULTS: A strong correlation between direct and transitive inference for significantly differentially expressed genes is demonstrated, using subsets of a dye-swap loop design. CONCLUSIONS: In experimental design, opportunities for transitive inference should be exploited, while always ensuring that comparisons of greatest interest comprise direct hybridizations. |
format | Text |
id | pubmed-239860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-2398602003-11-04 Multifactorial experimental design and the transitivity of ratios with spotted DNA microarrays Townsend, Jeffrey P BMC Genomics Methodology Article BACKGROUND: Multifactorial experimental designs using DNA microarrays are becoming increasingly common, but the extent of the transitivity of cDNA microarray expression measurements across multiple samples has yet to be explored. RESULTS: A strong correlation between direct and transitive inference for significantly differentially expressed genes is demonstrated, using subsets of a dye-swap loop design. CONCLUSIONS: In experimental design, opportunities for transitive inference should be exploited, while always ensuring that comparisons of greatest interest comprise direct hybridizations. BioMed Central 2003-10-02 /pmc/articles/PMC239860/ /pubmed/14525623 http://dx.doi.org/10.1186/1471-2164-4-41 Text en Copyright © 2003 Townsend; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Methodology Article Townsend, Jeffrey P Multifactorial experimental design and the transitivity of ratios with spotted DNA microarrays |
title | Multifactorial experimental design and the transitivity of ratios with spotted DNA microarrays |
title_full | Multifactorial experimental design and the transitivity of ratios with spotted DNA microarrays |
title_fullStr | Multifactorial experimental design and the transitivity of ratios with spotted DNA microarrays |
title_full_unstemmed | Multifactorial experimental design and the transitivity of ratios with spotted DNA microarrays |
title_short | Multifactorial experimental design and the transitivity of ratios with spotted DNA microarrays |
title_sort | multifactorial experimental design and the transitivity of ratios with spotted dna microarrays |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC239860/ https://www.ncbi.nlm.nih.gov/pubmed/14525623 http://dx.doi.org/10.1186/1471-2164-4-41 |
work_keys_str_mv | AT townsendjeffreyp multifactorialexperimentaldesignandthetransitivityofratioswithspotteddnamicroarrays |