New Molecular Reporters for Rapid Protein Folding Assays

The GFP folding reporter assay [1] uses a C-terminal GFP fusion to report on the folding success of upstream fused polypeptides. The GFP folding assay is widely-used for screening protein variants with improved folding and solubility [2]–[8], but truncation artifacts may arise during evolution, i.e....

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Detalles Bibliográficos
Autores principales: Cabantous, Stéphanie, Rogers, Yvonne, Terwilliger, Thomas C., Waldo, Geoffrey S.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408556/
https://www.ncbi.nlm.nih.gov/pubmed/18545698
http://dx.doi.org/10.1371/journal.pone.0002387

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408556/
https://www.ncbi.nlm.nih.gov/pubmed/18545698
http://dx.doi.org/10.1371/journal.pone.0002387

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