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PCR-based gene synthesis to produce recombinant proteins for crystallization

BACKGROUND: Gene synthesis technologies are an important tool for structural biology projects, allowing increased protein expression through codon optimization and facilitating sequence alterations. Existing methods, however, can be complex and not always reproducible, prompting researchers to use c...

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Detalles Bibliográficos
Autores principales:	Marsic, Damien, Hughes, Ronny C, Byrne-Steele, Miranda L, Ng, Joseph D
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2008
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408586/ https://www.ncbi.nlm.nih.gov/pubmed/18445293 http://dx.doi.org/10.1186/1472-6750-8-44

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