Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis

The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+)Msh2(+/–)and Msh2(–/–)mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction...

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Detalles Bibliográficos
Autores principales: Bouffler, S D, Hofland, N, Cox, R, Fodde, R
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408782/
https://www.ncbi.nlm.nih.gov/pubmed/11044352
http://dx.doi.org/10.1054/bjoc.2000.1422
Descripción
Sumario:The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+)Msh2(+/–)and Msh2(–/–)mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(–/–)and Msh2(+/–)cells to ~20% and ~45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. © 2000 CancerResearch Campaign

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