Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis

The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+)Msh2(+/–)and Msh2(–/–)mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction...

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Autores principales: Bouffler, S D, Hofland, N, Cox, R, Fodde, R
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408782/
https://www.ncbi.nlm.nih.gov/pubmed/11044352
http://dx.doi.org/10.1054/bjoc.2000.1422
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author Bouffler, S D
Hofland, N
Cox, R
Fodde, R
author_facet Bouffler, S D
Hofland, N
Cox, R
Fodde, R
author_sort Bouffler, S D
collection PubMed
description The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+)Msh2(+/–)and Msh2(–/–)mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(–/–)and Msh2(+/–)cells to ~20% and ~45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. © 2000 CancerResearch Campaign
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spelling pubmed-24087822009-09-10 Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis Bouffler, S D Hofland, N Cox, R Fodde, R Br J Cancer Short Communication The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+)Msh2(+/–)and Msh2(–/–)mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(–/–)and Msh2(+/–)cells to ~20% and ~45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. © 2000 CancerResearch Campaign Nature Publishing Group 2000-11 2000-10-26 /pmc/articles/PMC2408782/ /pubmed/11044352 http://dx.doi.org/10.1054/bjoc.2000.1422 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Short Communication
Bouffler, S D
Hofland, N
Cox, R
Fodde, R
Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis
title Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis
title_full Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis
title_fullStr Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis
title_full_unstemmed Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis
title_short Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis
title_sort evidence for msh2 haploinsufficiency in mice revealed by mnu-induced sister-chromatid exchange analysis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408782/
https://www.ncbi.nlm.nih.gov/pubmed/11044352
http://dx.doi.org/10.1054/bjoc.2000.1422
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