Cargando…

Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients

BACKGROUND: Recent attempts to diminish nickel use in most industrial products have led to an increasing utilization of alternative metal compounds for destinations such as the alloys used in orthopaedics, jewellery and dentistry. The present study was undertaken with the aim to evaluate the potenti...

Descripción completa

Detalles Bibliográficos
Autores principales: Bordignon, Valentina, Palamara, Francesca, Cordiali-Fei, Paola, Vento, Antonella, Aiello, Arianna, Picardo, Mauro, Ensoli, Fabrizio, Cristaudo, Antonio
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2409297/
https://www.ncbi.nlm.nih.gov/pubmed/18482439
http://dx.doi.org/10.1186/1471-2172-9-19
_version_ 1782155741202743296
author Bordignon, Valentina
Palamara, Francesca
Cordiali-Fei, Paola
Vento, Antonella
Aiello, Arianna
Picardo, Mauro
Ensoli, Fabrizio
Cristaudo, Antonio
author_facet Bordignon, Valentina
Palamara, Francesca
Cordiali-Fei, Paola
Vento, Antonella
Aiello, Arianna
Picardo, Mauro
Ensoli, Fabrizio
Cristaudo, Antonio
author_sort Bordignon, Valentina
collection PubMed
description BACKGROUND: Recent attempts to diminish nickel use in most industrial products have led to an increasing utilization of alternative metal compounds for destinations such as the alloys used in orthopaedics, jewellery and dentistry. The present study was undertaken with the aim to evaluate the potential for an allergic response to nickel, palladium and rhodium on the basis of antigen-specific induction of inflammatory/regulatory cytokines, and to characterize, according to the cytokine profiles, the nature of simultaneous positive patch tests elicited in vivo. Peripheral blood mononuclear cells (PBMC) from 40 patients with different patch test results were kept in short term cultures in the presence of optimized concentrations of NiSO(4 )× 6H(2)O, PdCl(2 )and Rh(CH(3)COO)(2). The production of IFN-γ and IL-10 elicited by metal compounds were analyzed by the ELISpot assay. RESULTS: We found a specific IFN-γ response by PBMC upon in vitro stimulation with nickel or palladium in well recognized allergic individuals. All controls with a negative patch test to a metal salt showed an in vitro IL-10 response and not IFN-γ production when challenged with the same compound. Interestingly, all subjects with positive patch test to both nickel and palladium (group 3) showed an in vitro response characterized by the release of IFN-γ after nickel stimulation and production of IL-10 in response to palladium. CONCLUSION: These results strongly suggest that the different cytokine profiles elicited in vitro reflect different immune responses which may lead to the control of the allergic responses or to symptomatic allergic contact dermatitis. The development of sensitive and specific in vitro assays based on the determination of the cytokine profiles in response to contact allergens may have important diagnostic and prognostic implications and may prove extremely useful in complementing the diagnostic limits of traditional patch testing.
format Text
id pubmed-2409297
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-24092972008-06-04 Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients Bordignon, Valentina Palamara, Francesca Cordiali-Fei, Paola Vento, Antonella Aiello, Arianna Picardo, Mauro Ensoli, Fabrizio Cristaudo, Antonio BMC Immunol Research Article BACKGROUND: Recent attempts to diminish nickel use in most industrial products have led to an increasing utilization of alternative metal compounds for destinations such as the alloys used in orthopaedics, jewellery and dentistry. The present study was undertaken with the aim to evaluate the potential for an allergic response to nickel, palladium and rhodium on the basis of antigen-specific induction of inflammatory/regulatory cytokines, and to characterize, according to the cytokine profiles, the nature of simultaneous positive patch tests elicited in vivo. Peripheral blood mononuclear cells (PBMC) from 40 patients with different patch test results were kept in short term cultures in the presence of optimized concentrations of NiSO(4 )× 6H(2)O, PdCl(2 )and Rh(CH(3)COO)(2). The production of IFN-γ and IL-10 elicited by metal compounds were analyzed by the ELISpot assay. RESULTS: We found a specific IFN-γ response by PBMC upon in vitro stimulation with nickel or palladium in well recognized allergic individuals. All controls with a negative patch test to a metal salt showed an in vitro IL-10 response and not IFN-γ production when challenged with the same compound. Interestingly, all subjects with positive patch test to both nickel and palladium (group 3) showed an in vitro response characterized by the release of IFN-γ after nickel stimulation and production of IL-10 in response to palladium. CONCLUSION: These results strongly suggest that the different cytokine profiles elicited in vitro reflect different immune responses which may lead to the control of the allergic responses or to symptomatic allergic contact dermatitis. The development of sensitive and specific in vitro assays based on the determination of the cytokine profiles in response to contact allergens may have important diagnostic and prognostic implications and may prove extremely useful in complementing the diagnostic limits of traditional patch testing. BioMed Central 2008-05-15 /pmc/articles/PMC2409297/ /pubmed/18482439 http://dx.doi.org/10.1186/1471-2172-9-19 Text en Copyright © 2008 Bordignon et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bordignon, Valentina
Palamara, Francesca
Cordiali-Fei, Paola
Vento, Antonella
Aiello, Arianna
Picardo, Mauro
Ensoli, Fabrizio
Cristaudo, Antonio
Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients
title Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients
title_full Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients
title_fullStr Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients
title_full_unstemmed Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients
title_short Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients
title_sort nickel, palladium and rhodium induced ifn-gamma and il-10 production as assessed by in vitro elispot-analysis in contact dermatitis patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2409297/
https://www.ncbi.nlm.nih.gov/pubmed/18482439
http://dx.doi.org/10.1186/1471-2172-9-19
work_keys_str_mv AT bordignonvalentina nickelpalladiumandrhodiuminducedifngammaandil10productionasassessedbyinvitroelispotanalysisincontactdermatitispatients
AT palamarafrancesca nickelpalladiumandrhodiuminducedifngammaandil10productionasassessedbyinvitroelispotanalysisincontactdermatitispatients
AT cordialifeipaola nickelpalladiumandrhodiuminducedifngammaandil10productionasassessedbyinvitroelispotanalysisincontactdermatitispatients
AT ventoantonella nickelpalladiumandrhodiuminducedifngammaandil10productionasassessedbyinvitroelispotanalysisincontactdermatitispatients
AT aielloarianna nickelpalladiumandrhodiuminducedifngammaandil10productionasassessedbyinvitroelispotanalysisincontactdermatitispatients
AT picardomauro nickelpalladiumandrhodiuminducedifngammaandil10productionasassessedbyinvitroelispotanalysisincontactdermatitispatients
AT ensolifabrizio nickelpalladiumandrhodiuminducedifngammaandil10productionasassessedbyinvitroelispotanalysisincontactdermatitispatients
AT cristaudoantonio nickelpalladiumandrhodiuminducedifngammaandil10productionasassessedbyinvitroelispotanalysisincontactdermatitispatients