Detection of HER-2/neu-positive circulating epithelial cells in prostate cancer patients

HER-2/neu may play a role in prostate carcinogenesis. The aim of this study was to use the expression of HER-2/neu as a molecular marker for the detection of circulating tumour cells (CTCs) in the blood of patients with prostate cancer (PC). Blood samples were collected from 42 patients with PC and...

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Autores principales: Ady, N, Morat, L, Fizazi, K, Soria, J-C, Mathieu, M-C, Prapotnich, D, Sabatier, L, Chauveinc, L
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2004
Materias:
Molecular and Cellular Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2409539/
https://www.ncbi.nlm.nih.gov/pubmed/14735191
http://dx.doi.org/10.1038/sj.bjc.6601532
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author Ady, N
Morat, L
Fizazi, K
Soria, J-C
Mathieu, M-C
Prapotnich, D
Sabatier, L
Chauveinc, L
author_facet Ady, N
Morat, L
Fizazi, K
Soria, J-C
Mathieu, M-C
Prapotnich, D
Sabatier, L
Chauveinc, L
author_sort Ady, N
collection PubMed
description HER-2/neu may play a role in prostate carcinogenesis. The aim of this study was to use the expression of HER-2/neu as a molecular marker for the detection of circulating tumour cells (CTCs) in the blood of patients with prostate cancer (PC). Blood samples were collected from 42 patients with PC and nine healthy volunteers. Immunomagnetic beads were used to harvest epithelial cells from peripheral blood mononuclear cells. Total RNA was extracted and reverse transcribed before analysis by real-time PCR with HER-2/neu-specific primers. CTCs were HER-2/neu positive in six out of 11 (54%) patients with metastatic disease and in three out of 31 (9.6%) patients with localised PC (P=0.004). In blood samples from nine healthy volunteers, we detected no expression of HER-2/neu. The present method appears to be minimally invasive, highly sensitive and a specific approach for detecting CTCs in PC. Furthermore, it may help better target HER-2/neu in advanced PC.
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spelling pubmed-24095392009-09-10 Detection of HER-2/neu-positive circulating epithelial cells in prostate cancer patients Ady, N Morat, L Fizazi, K Soria, J-C Mathieu, M-C Prapotnich, D Sabatier, L Chauveinc, L Br J Cancer Molecular and Cellular Pathology HER-2/neu may play a role in prostate carcinogenesis. The aim of this study was to use the expression of HER-2/neu as a molecular marker for the detection of circulating tumour cells (CTCs) in the blood of patients with prostate cancer (PC). Blood samples were collected from 42 patients with PC and nine healthy volunteers. Immunomagnetic beads were used to harvest epithelial cells from peripheral blood mononuclear cells. Total RNA was extracted and reverse transcribed before analysis by real-time PCR with HER-2/neu-specific primers. CTCs were HER-2/neu positive in six out of 11 (54%) patients with metastatic disease and in three out of 31 (9.6%) patients with localised PC (P=0.004). In blood samples from nine healthy volunteers, we detected no expression of HER-2/neu. The present method appears to be minimally invasive, highly sensitive and a specific approach for detecting CTCs in PC. Furthermore, it may help better target HER-2/neu in advanced PC. Nature Publishing Group 2004-01-26 2004-01-20 /pmc/articles/PMC2409539/ /pubmed/14735191 http://dx.doi.org/10.1038/sj.bjc.6601532 Text en Copyright © 2004 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular and Cellular Pathology
Ady, N
Morat, L
Fizazi, K
Soria, J-C
Mathieu, M-C
Prapotnich, D
Sabatier, L
Chauveinc, L
Detection of HER-2/neu-positive circulating epithelial cells in prostate cancer patients
title Detection of HER-2/neu-positive circulating epithelial cells in prostate cancer patients
title_full Detection of HER-2/neu-positive circulating epithelial cells in prostate cancer patients
title_fullStr Detection of HER-2/neu-positive circulating epithelial cells in prostate cancer patients
title_full_unstemmed Detection of HER-2/neu-positive circulating epithelial cells in prostate cancer patients
title_short Detection of HER-2/neu-positive circulating epithelial cells in prostate cancer patients
title_sort detection of her-2/neu-positive circulating epithelial cells in prostate cancer patients
topic Molecular and Cellular Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2409539/
https://www.ncbi.nlm.nih.gov/pubmed/14735191
http://dx.doi.org/10.1038/sj.bjc.6601532
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