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Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)

Diagnosis of malignant cells in effusions is important for staging procedures and resulting therapeutic decisions. Cytodiagnostics in effusions is sometimes difficult since reactive mesothelial cells can mimic malignant cells. We used fluorescence in situ hybridisation (FISH) in single-colour or if...

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Autores principales: Fiegl, M, Massoner, A, Haun, M, Sturm, W, Kaufmann, H, Hack, R, Krugmann, J, Fritzer-Szekeres, M, Grünewald, K, Gastl, G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2409837/
https://www.ncbi.nlm.nih.gov/pubmed/15226776
http://dx.doi.org/10.1038/sj.bjc.6601942
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author Fiegl, M
Massoner, A
Haun, M
Sturm, W
Kaufmann, H
Hack, R
Krugmann, J
Fritzer-Szekeres, M
Grünewald, K
Gastl, G
author_facet Fiegl, M
Massoner, A
Haun, M
Sturm, W
Kaufmann, H
Hack, R
Krugmann, J
Fritzer-Szekeres, M
Grünewald, K
Gastl, G
author_sort Fiegl, M
collection PubMed
description Diagnosis of malignant cells in effusions is important for staging procedures and resulting therapeutic decisions. Cytodiagnostics in effusions is sometimes difficult since reactive mesothelial cells can mimic malignant cells. We used fluorescence in situ hybridisation (FISH) in single-colour or if appropriate in dual-colour evaluation to detect chromosomal aberrations in effusion cells as markers of malignancy, to raise the diagnostic yield. Cytologic and FISH evaluations – by using probes representing several chromosomes always including chromosomes 11 and 17 – were performed in 358 effusion fluids. Cytology was positive for malignancy in 44.4% of all effusions, whereas FISH was positive in 53.9% (P=0.0001). The combination of cytology and FISH was diagnostic for malignancy in 60.9% of effusions. Diagnostic superiority of FISH was demonstrated in effusions from breast cancer, lung cancer, pancreatic cancer, and in effusions from the entire group of gynaecological and gastrointestinal carcinomas. In transudates (effusion protein <2.5 g dl(−1)), malignant cells were detectable by cytology, FISH, and combined use of both methods in 18.6, 30, and 37.1% of effusions, respectively, suggesting that cytologic and molecular analysis should be performed also with transudates. In conclusion, FISH in combination with conventional cytology is a highly sensitive and specific diagnostic tool for detecting malignant cells in effusions.
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spelling pubmed-24098372009-09-10 Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH) Fiegl, M Massoner, A Haun, M Sturm, W Kaufmann, H Hack, R Krugmann, J Fritzer-Szekeres, M Grünewald, K Gastl, G Br J Cancer Molecular and Cellular Pathology Diagnosis of malignant cells in effusions is important for staging procedures and resulting therapeutic decisions. Cytodiagnostics in effusions is sometimes difficult since reactive mesothelial cells can mimic malignant cells. We used fluorescence in situ hybridisation (FISH) in single-colour or if appropriate in dual-colour evaluation to detect chromosomal aberrations in effusion cells as markers of malignancy, to raise the diagnostic yield. Cytologic and FISH evaluations – by using probes representing several chromosomes always including chromosomes 11 and 17 – were performed in 358 effusion fluids. Cytology was positive for malignancy in 44.4% of all effusions, whereas FISH was positive in 53.9% (P=0.0001). The combination of cytology and FISH was diagnostic for malignancy in 60.9% of effusions. Diagnostic superiority of FISH was demonstrated in effusions from breast cancer, lung cancer, pancreatic cancer, and in effusions from the entire group of gynaecological and gastrointestinal carcinomas. In transudates (effusion protein <2.5 g dl(−1)), malignant cells were detectable by cytology, FISH, and combined use of both methods in 18.6, 30, and 37.1% of effusions, respectively, suggesting that cytologic and molecular analysis should be performed also with transudates. In conclusion, FISH in combination with conventional cytology is a highly sensitive and specific diagnostic tool for detecting malignant cells in effusions. Nature Publishing Group 2004-08-02 2004-06-29 /pmc/articles/PMC2409837/ /pubmed/15226776 http://dx.doi.org/10.1038/sj.bjc.6601942 Text en Copyright © 2004 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular and Cellular Pathology
Fiegl, M
Massoner, A
Haun, M
Sturm, W
Kaufmann, H
Hack, R
Krugmann, J
Fritzer-Szekeres, M
Grünewald, K
Gastl, G
Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)
title Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)
title_full Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)
title_fullStr Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)
title_full_unstemmed Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)
title_short Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)
title_sort sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (fish)
topic Molecular and Cellular Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2409837/
https://www.ncbi.nlm.nih.gov/pubmed/15226776
http://dx.doi.org/10.1038/sj.bjc.6601942
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