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Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells
To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The m...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2410236/ https://www.ncbi.nlm.nih.gov/pubmed/14960165 http://dx.doi.org/10.1186/1476-5926-2-S1-S13 |
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author | Imai, Katsuyuki Sato, Mitsuru Sato, Takeya Kojima, Naosuke Miura, Mitsutaka Higashi, Nobuyo Wang, Da-Ren Suzuki, Shinsuke Senoo, Haruki |
author_facet | Imai, Katsuyuki Sato, Mitsuru Sato, Takeya Kojima, Naosuke Miura, Mitsutaka Higashi, Nobuyo Wang, Da-Ren Suzuki, Shinsuke Senoo, Haruki |
author_sort | Imai, Katsuyuki |
collection | PubMed |
description | To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. |
format | Text |
id | pubmed-2410236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-24102362008-06-05 Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells Imai, Katsuyuki Sato, Mitsuru Sato, Takeya Kojima, Naosuke Miura, Mitsutaka Higashi, Nobuyo Wang, Da-Ren Suzuki, Shinsuke Senoo, Haruki Comp Hepatol Proceedings To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. BioMed Central 2004-01-14 /pmc/articles/PMC2410236/ /pubmed/14960165 http://dx.doi.org/10.1186/1476-5926-2-S1-S13 Text en Copyright © 2004 Imai et al; licensee BioMed Central Ltd This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Proceedings Imai, Katsuyuki Sato, Mitsuru Sato, Takeya Kojima, Naosuke Miura, Mitsutaka Higashi, Nobuyo Wang, Da-Ren Suzuki, Shinsuke Senoo, Haruki Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells |
title | Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells |
title_full | Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells |
title_fullStr | Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells |
title_full_unstemmed | Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells |
title_short | Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells |
title_sort | intercellular adhesive structures between stellate cells – an analysis in cultured human hepatic stellate cells |
topic | Proceedings |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2410236/ https://www.ncbi.nlm.nih.gov/pubmed/14960165 http://dx.doi.org/10.1186/1476-5926-2-S1-S13 |
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