Cargando…
Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells
We investigated activation mechanisms of hepatic stellate cells (HSCs) that are known to play pivotal roles in the regeneration process after 70% partial hepatectomy (PHx). Parenchymal liver cells (PLCs) and non-parenchymal cells (NPLCs) were isolated and purified from the regenerating livers at 1,...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2004
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2410248/ https://www.ncbi.nlm.nih.gov/pubmed/14960181 http://dx.doi.org/10.1186/1476-5926-2-S1-S29 |
_version_ | 1782155960045797376 |
---|---|
author | Mabuchi, Ayako Mullaney, Ian Sheard, Philip Hessian, Paul Zimmermann, Arthur Senoo, Haruki Wheatley, Antony M |
author_facet | Mabuchi, Ayako Mullaney, Ian Sheard, Philip Hessian, Paul Zimmermann, Arthur Senoo, Haruki Wheatley, Antony M |
author_sort | Mabuchi, Ayako |
collection | PubMed |
description | We investigated activation mechanisms of hepatic stellate cells (HSCs) that are known to play pivotal roles in the regeneration process after 70% partial hepatectomy (PHx). Parenchymal liver cells (PLCs) and non-parenchymal cells (NPLCs) were isolated and purified from the regenerating livers at 1, 3, 7, 14 days after PHx. Each liver cell fraction was stained by immunocytochemistry using an anti-desmin antibody as a marker for HSCs, anti-alpha-smooth muscle actin (alpha-SMA) as a marker for activated HSCs, and 5-bromo-2'-deoxyuridine (BrdU) for detection of proliferating cells. Tissue sections from regenerating livers were also analyzed by immunohistochemistry and compared with the results obtained for isolated cell fractions. One and 3 days after PHx, PLC-enriched fraction contained HSCs adhered to PLCs. The HSCs adhered to PLCs were double positive for BrdU and alpha-SMA, and formed clusters suggesting that these HSCs were activated. However, HSC-enriched fraction contained HSCs not adhered PLCs showed positive staining for anti-desmin antibody but negative for anti-alpha-SMA antibody. These results suggest that HSCs are activated by adhering to PLCs during the early phase of hepatic regeneration. |
format | Text |
id | pubmed-2410248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-24102482008-06-05 Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells Mabuchi, Ayako Mullaney, Ian Sheard, Philip Hessian, Paul Zimmermann, Arthur Senoo, Haruki Wheatley, Antony M Comp Hepatol Proceedings We investigated activation mechanisms of hepatic stellate cells (HSCs) that are known to play pivotal roles in the regeneration process after 70% partial hepatectomy (PHx). Parenchymal liver cells (PLCs) and non-parenchymal cells (NPLCs) were isolated and purified from the regenerating livers at 1, 3, 7, 14 days after PHx. Each liver cell fraction was stained by immunocytochemistry using an anti-desmin antibody as a marker for HSCs, anti-alpha-smooth muscle actin (alpha-SMA) as a marker for activated HSCs, and 5-bromo-2'-deoxyuridine (BrdU) for detection of proliferating cells. Tissue sections from regenerating livers were also analyzed by immunohistochemistry and compared with the results obtained for isolated cell fractions. One and 3 days after PHx, PLC-enriched fraction contained HSCs adhered to PLCs. The HSCs adhered to PLCs were double positive for BrdU and alpha-SMA, and formed clusters suggesting that these HSCs were activated. However, HSC-enriched fraction contained HSCs not adhered PLCs showed positive staining for anti-desmin antibody but negative for anti-alpha-SMA antibody. These results suggest that HSCs are activated by adhering to PLCs during the early phase of hepatic regeneration. BioMed Central 2004-01-14 /pmc/articles/PMC2410248/ /pubmed/14960181 http://dx.doi.org/10.1186/1476-5926-2-S1-S29 Text en Copyright © 2004 Mabuchi et al; licensee BioMed Central Ltd This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Proceedings Mabuchi, Ayako Mullaney, Ian Sheard, Philip Hessian, Paul Zimmermann, Arthur Senoo, Haruki Wheatley, Antony M Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells |
title | Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells |
title_full | Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells |
title_fullStr | Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells |
title_full_unstemmed | Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells |
title_short | Role of Hepatic Stellate Cells in the Early Phase of Liver Regeneration in Rat: Formation of Tight Adhesion to Parenchymal Cells |
title_sort | role of hepatic stellate cells in the early phase of liver regeneration in rat: formation of tight adhesion to parenchymal cells |
topic | Proceedings |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2410248/ https://www.ncbi.nlm.nih.gov/pubmed/14960181 http://dx.doi.org/10.1186/1476-5926-2-S1-S29 |
work_keys_str_mv | AT mabuchiayako roleofhepaticstellatecellsintheearlyphaseofliverregenerationinratformationoftightadhesiontoparenchymalcells AT mullaneyian roleofhepaticstellatecellsintheearlyphaseofliverregenerationinratformationoftightadhesiontoparenchymalcells AT sheardphilip roleofhepaticstellatecellsintheearlyphaseofliverregenerationinratformationoftightadhesiontoparenchymalcells AT hessianpaul roleofhepaticstellatecellsintheearlyphaseofliverregenerationinratformationoftightadhesiontoparenchymalcells AT zimmermannarthur roleofhepaticstellatecellsintheearlyphaseofliverregenerationinratformationoftightadhesiontoparenchymalcells AT senooharuki roleofhepaticstellatecellsintheearlyphaseofliverregenerationinratformationoftightadhesiontoparenchymalcells AT wheatleyantonym roleofhepaticstellatecellsintheearlyphaseofliverregenerationinratformationoftightadhesiontoparenchymalcells |