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Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus
In this work, a recombinant plum pox virus (PPV, Sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and protein expression at the subcellular level in pea plants (cv. Alaska). PPV had produced chlorotic spots as well as necrotic spots in the oldest leaves a...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2413280/ https://www.ncbi.nlm.nih.gov/pubmed/18535298 http://dx.doi.org/10.1093/jxb/ern082 |
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author | Díaz-Vivancos, Pedro Clemente-Moreno, María José Rubio, Manuel Olmos, Enrique García, Juan Antonio Martínez-Gómez, Pedro Hernández, José Antonio |
author_facet | Díaz-Vivancos, Pedro Clemente-Moreno, María José Rubio, Manuel Olmos, Enrique García, Juan Antonio Martínez-Gómez, Pedro Hernández, José Antonio |
author_sort | Díaz-Vivancos, Pedro |
collection | PubMed |
description | In this work, a recombinant plum pox virus (PPV, Sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and protein expression at the subcellular level in pea plants (cv. Alaska). PPV had produced chlorotic spots as well as necrotic spots in the oldest leaves at 13–15 d post-inoculation. At 15 d post-inoculation, PPV was present in the chlorotic and necrotic areas, as shown by the fluorescence signal produced by the presence of the green fluorescent protein. In the same areas, an accumulation of reactive oxygen species was noticed. Studies with laser confocal and electron microscopy demonstrated that PPV accumulated in the cytosol of infected cells. In addition, PPV infection produced an alteration in the chloroplast ultrastructure, giving rise to dilated thylakoids, an increase in the number of plastoglobuli, and a decreased amount of starch content. At 3 d post-inoculation, although no changes in the oxidative stress parameters were observed, an increase in the chloroplastic hydrogen peroxide levels was observed that correlated with a decrease in the enzymatic mechanisms involved in its elimination (ascorbate peroxidase and peroxidase) in this cell compartment. These results indicate that an alteration in the chloroplastic metabolism is produced in the early response to PPV. This oxidative stress is more pronounced during the development of the disease (15 d post-inoculation) judging from the increase in oxidative stress parameters as well as the imbalance in the antioxidative systems, mainly at the chloroplastic level. Finally, proteomic analyses showed that most of the changes produced by PPV infection with regard to protein expression at the subcellular level were related mainly to photosynthesis and carbohydrate metabolism. It seems that PPV infection has some effect on PSII, directly or indirectly, by decreasing the amount of Rubisco, oxygen-evolving enhancer, and PSII stability factor proteins. The results indicate that Sharka symptoms observed in pea leaves could be due to an imbalance in antioxidant systems as well as to an increased generation of reactive oxygen species in chloroplasts, induced probably by a disturbance of the electron transport chain, suggesting that chloroplasts can be a source of oxidative stress during viral disease development. |
format | Text |
id | pubmed-2413280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-24132802009-02-25 Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus Díaz-Vivancos, Pedro Clemente-Moreno, María José Rubio, Manuel Olmos, Enrique García, Juan Antonio Martínez-Gómez, Pedro Hernández, José Antonio J Exp Bot Research Papers In this work, a recombinant plum pox virus (PPV, Sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and protein expression at the subcellular level in pea plants (cv. Alaska). PPV had produced chlorotic spots as well as necrotic spots in the oldest leaves at 13–15 d post-inoculation. At 15 d post-inoculation, PPV was present in the chlorotic and necrotic areas, as shown by the fluorescence signal produced by the presence of the green fluorescent protein. In the same areas, an accumulation of reactive oxygen species was noticed. Studies with laser confocal and electron microscopy demonstrated that PPV accumulated in the cytosol of infected cells. In addition, PPV infection produced an alteration in the chloroplast ultrastructure, giving rise to dilated thylakoids, an increase in the number of plastoglobuli, and a decreased amount of starch content. At 3 d post-inoculation, although no changes in the oxidative stress parameters were observed, an increase in the chloroplastic hydrogen peroxide levels was observed that correlated with a decrease in the enzymatic mechanisms involved in its elimination (ascorbate peroxidase and peroxidase) in this cell compartment. These results indicate that an alteration in the chloroplastic metabolism is produced in the early response to PPV. This oxidative stress is more pronounced during the development of the disease (15 d post-inoculation) judging from the increase in oxidative stress parameters as well as the imbalance in the antioxidative systems, mainly at the chloroplastic level. Finally, proteomic analyses showed that most of the changes produced by PPV infection with regard to protein expression at the subcellular level were related mainly to photosynthesis and carbohydrate metabolism. It seems that PPV infection has some effect on PSII, directly or indirectly, by decreasing the amount of Rubisco, oxygen-evolving enhancer, and PSII stability factor proteins. The results indicate that Sharka symptoms observed in pea leaves could be due to an imbalance in antioxidant systems as well as to an increased generation of reactive oxygen species in chloroplasts, induced probably by a disturbance of the electron transport chain, suggesting that chloroplasts can be a source of oxidative stress during viral disease development. Oxford University Press 2008-05 /pmc/articles/PMC2413280/ /pubmed/18535298 http://dx.doi.org/10.1093/jxb/ern082 Text en © 2008 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details) |
spellingShingle | Research Papers Díaz-Vivancos, Pedro Clemente-Moreno, María José Rubio, Manuel Olmos, Enrique García, Juan Antonio Martínez-Gómez, Pedro Hernández, José Antonio Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus |
title | Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus |
title_full | Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus |
title_fullStr | Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus |
title_full_unstemmed | Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus |
title_short | Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus |
title_sort | alteration in the chloroplastic metabolism leads to ros accumulation in pea plants in response to plum pox virus |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2413280/ https://www.ncbi.nlm.nih.gov/pubmed/18535298 http://dx.doi.org/10.1093/jxb/ern082 |
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