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Cdk5-mediated Phosphorylation of c-Myc on Ser-62 Is Essential in Transcriptional Activation of Cyclin B1 by Cyclin G1
It has been reported previously that cyclin G1 enables cells to overcome radiation-induced G(2) arrest and increased cell death and that these effects are mediated by transcriptional activation of cyclin B1. In this study, we further investigated the mechanism by which cyclin G1 transcriptionally ac...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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American Society for Biochemistry and Molecular Biology
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2414302/ https://www.ncbi.nlm.nih.gov/pubmed/18408012 http://dx.doi.org/10.1074/jbc.M800987200 |
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author | Seo, Haeng Ran Kim, Joon Bae, Sangwoo Soh, Jae-Won Lee, Yun-Sil |
author_facet | Seo, Haeng Ran Kim, Joon Bae, Sangwoo Soh, Jae-Won Lee, Yun-Sil |
author_sort | Seo, Haeng Ran |
collection | PubMed |
description | It has been reported previously that cyclin G1 enables cells to overcome radiation-induced G(2) arrest and increased cell death and that these effects are mediated by transcriptional activation of cyclin B1. In this study, we further investigated the mechanism by which cyclin G1 transcriptionally activates cyclin B1. Deletion or point mutations within the cyclin B1 promoter region revealed that the c-Myc binding site (E-box) is necessary for cyclin G1-mediated transcriptional activation of cyclin B1 to occur. In addition, the kinase activity of Cdk5 was increased by cyclin G1 overexpression, and Cdk5 directly phosphorylated c-Myc on Ser-62. Furthermore, cyclin G1 mediated increased radiosensitivity, and radiation-induced M phase arrest was attenuated when RNA interference of Cdk5 was treated. Taken together, the results of this study indicate that Cdk5 activation in cells that overexpress cyclin G1 leads to c-Myc phosphorylation on Ser-62, which is responsible for cyclin G1-mediated transcriptional activation of cyclin B1. |
format | Text |
id | pubmed-2414302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-24143022008-09-18 Cdk5-mediated Phosphorylation of c-Myc on Ser-62 Is Essential in Transcriptional Activation of Cyclin B1 by Cyclin G1 Seo, Haeng Ran Kim, Joon Bae, Sangwoo Soh, Jae-Won Lee, Yun-Sil J Biol Chem Mechanisms of Signal Transduction It has been reported previously that cyclin G1 enables cells to overcome radiation-induced G(2) arrest and increased cell death and that these effects are mediated by transcriptional activation of cyclin B1. In this study, we further investigated the mechanism by which cyclin G1 transcriptionally activates cyclin B1. Deletion or point mutations within the cyclin B1 promoter region revealed that the c-Myc binding site (E-box) is necessary for cyclin G1-mediated transcriptional activation of cyclin B1 to occur. In addition, the kinase activity of Cdk5 was increased by cyclin G1 overexpression, and Cdk5 directly phosphorylated c-Myc on Ser-62. Furthermore, cyclin G1 mediated increased radiosensitivity, and radiation-induced M phase arrest was attenuated when RNA interference of Cdk5 was treated. Taken together, the results of this study indicate that Cdk5 activation in cells that overexpress cyclin G1 leads to c-Myc phosphorylation on Ser-62, which is responsible for cyclin G1-mediated transcriptional activation of cyclin B1. American Society for Biochemistry and Molecular Biology 2008-06-06 /pmc/articles/PMC2414302/ /pubmed/18408012 http://dx.doi.org/10.1074/jbc.M800987200 Text en Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles |
spellingShingle | Mechanisms of Signal Transduction Seo, Haeng Ran Kim, Joon Bae, Sangwoo Soh, Jae-Won Lee, Yun-Sil Cdk5-mediated Phosphorylation of c-Myc on Ser-62 Is Essential in Transcriptional Activation of Cyclin B1 by Cyclin G1 |
title | Cdk5-mediated Phosphorylation of c-Myc on Ser-62 Is Essential in
Transcriptional Activation of Cyclin B1 by Cyclin
G1 |
title_full | Cdk5-mediated Phosphorylation of c-Myc on Ser-62 Is Essential in
Transcriptional Activation of Cyclin B1 by Cyclin
G1 |
title_fullStr | Cdk5-mediated Phosphorylation of c-Myc on Ser-62 Is Essential in
Transcriptional Activation of Cyclin B1 by Cyclin
G1 |
title_full_unstemmed | Cdk5-mediated Phosphorylation of c-Myc on Ser-62 Is Essential in
Transcriptional Activation of Cyclin B1 by Cyclin
G1 |
title_short | Cdk5-mediated Phosphorylation of c-Myc on Ser-62 Is Essential in
Transcriptional Activation of Cyclin B1 by Cyclin
G1 |
title_sort | cdk5-mediated phosphorylation of c-myc on ser-62 is essential in
transcriptional activation of cyclin b1 by cyclin
g1 |
topic | Mechanisms of Signal Transduction |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2414302/ https://www.ncbi.nlm.nih.gov/pubmed/18408012 http://dx.doi.org/10.1074/jbc.M800987200 |
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